Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/22465
Title: | The angiogenic potential of leukocyte- and platelet-rich fibrin | Authors: | Berkmans, Kirsten | Advisors: | BRONCKAERS, Annelies LAMBRICHTS, Ivo |
Issue Date: | 2016 | Publisher: | tUL | Abstract: | Introduction: L-PRF promotes tissue healing in clinical applications such as dental surgery. However, little is known about the mechanisms of action. Angiogenesis is a key process in tissue regeneration. Growth factors, fibrin and leukocytes, as present in L-PRF, are known to be important in angiogenesis. The goal of this study was to investigate whether L-PRF induces blood vessel formation. Materials and methods: The angiogenic cytokine profile of L-PRF CM and exudate was determined by an antibody array. The effect of L-PRF, and the involvement of CXCR2, on HUVEC viability, migration and tube formation was examined in vitro. Surface expression of CXCR2 on HUVEC was verified by flow cytometry. The ability of L-PRF to induce blood vessel formation in ovo was assessed. Results: An upregulation of pro-angiogenic factors that bind to CXCR2 was observed in CM. L-PRF was able to enhance in vitro HUVEC viability, migration and tube formation, although the CXCR2 antagonist SB225002 did not attenuate these effects. Flow cytometry showed that a small percentage of HUVEC expresses CXCR2. The pro-angiogenic effect of L-PRF was confirmed in the CAM assay. Conclusion: L-PRF exerts pro-angiogenic effects in vitro and in ovo. CXCR2 was not found as a main mediator of this enhancement. A better insight in the mechanisms of action of L-PRF is required to better understand its clinical effects and its potential as a strategy for therapeutic angiogenesis. | Notes: | master in de biomedische wetenschappen-klinische moleculaire wetenschappen | Document URI: | http://hdl.handle.net/1942/22465 | Category: | T2 | Type: | Theses and Dissertations |
Appears in Collections: | Master theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
11318582015249.pdf | 1.02 MB | Adobe PDF | View/Open |
Page view(s)
26
checked on Oct 29, 2023
Download(s)
12
checked on Oct 29, 2023
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.