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Title: | Cardiac atrial appendage stem cells promote angiogenesis in vitro and in vivo | Authors: | FANTON, Yanick Houbrechts, Cynthia WILLEMS, Leen Daniëls, Annick LINSEN, Loes RATAJCZAK, Jessica BRONCKAERS, Annelies LAMBRICHTS, Ivo DECLERCQ, Jeroen RUMMENS, Jean-Luc HENDRIKX, Marc HENSEN, Karen |
Issue Date: | 2016 | Publisher: | ELSEVIER SCI LTD | Source: | JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 97, p. 235-244 | Abstract: | Cardiac atrial appendage stem cells (CASCs) show extraordinary myocardial differentiation properties, making them ideal candidates for myocardial regeneration. However, since the myocardium is a highly vascularized tissue, revascularization of the ischemic infarct area is essential for functional repair. Therefore, this study assessed if CASCs contribute to cardiac angiogenesis via paracrine mechanisms. First, it was demonstrated that CASCs produce and secrete high levels of numerous angiogenic growth factors, including vascular endothelial growth factor (VEGF), endothelin-1 (ET-1) and insulin-like growth factor binding protein 3 (IGFBP-3). Functional in vitro assays with a human microvascular endothelial cell line (HMEC-1) and CASC CM showed that CASCs promote endothelial cell proliferation, migration and tube formation, the most important steps of the angiogenesis process. Addition of inhibitory antibodies against identified growth factors could significantly reduce these effects, indicating their importance in CASC-induced neovascularization. The angiogenic potential of CASCs and CASC CM was also confirmed in a chorioallantoic membrane assay, demonstrating that CASCs promote blood vessel formation in vivo. In conclusion, this study shows that CASCs not only induce myocardial repair by cardiomyogenic differentiation, but also stimulate blood vessel formation by paracrine mechanisms. The angiogenic properties of CASCs further strengthen their therapeutic potential and make them an optimal stem cell source for the treatment of ischemic heart disease. | Notes: | [Fanton, Yanick; Willems, Leen; Daniels, Annick; Declercq, Jeroen; Rummens, Jean-Luc; Hensen, Karen] Jessa Hosp, Lab Expt Hematol, Hasselt, Belgium. [Fanton, Yanick; Houbrechts, Cynthia; Willems, Leen; Declercq, Jeroen; Rummens, Jean-Luc; Hendrikx, Marc; Hensen, Karen] Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium. [Linsen, Loes] Univ Hosp Leuven, AC Biobanking, Leuven, Belgium. [Ratajczak, Jessica; Bronckaers, Annelies; Lambrichts, Ivo] Hasselt Univ, Biomed Res Inst, Hasselt, Belgium. [Hendrikx, Marc] Jessa Hosp, Dept Cardiothorac Surg, Hasselt, Belgium. | Keywords: | angiogenesis; growth factors and cytokines; stem cell; cardiac progenitor cells; endothelial cell;Angiogenesis; Growth factors and cytokines; Stem cell; Cardiac progenitor cells; Endothelial cell | Document URI: | http://hdl.handle.net/1942/22595 | ISSN: | 0022-2828 | e-ISSN: | 1095-8584 | DOI: | 10.1016/j.yjmcc.2016.06.005 | ISI #: | 000382800100026 | Rights: | (C) 2016 Elsevier Ltd. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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fanton 1.pdf Restricted Access | Published version | 2.24 MB | Adobe PDF | View/Open Request a copy |
Cardiac.pdf | Peer-reviewed author version | 1.1 MB | Adobe PDF | View/Open |
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