Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/22717
Title: | A proposed integrated approach for the preclinical evaluation of phage therapy in Pseudomonas infections | Authors: | Danis-Wlodarczyk, Katarzyna Vandenheuvel, Dieter Bin Jang, Ho Briers, Yves Olszak, Tomasz Arabski, Michal Wasik, Slawomir Drabik, Marcin Higgins, Gerard Tyrrell, Jean Harvey, Brian J. NOBEN, Jean-Paul Lavigne, Rob Drulis-Kawa, Zuzanna |
Issue Date: | 2016 | Publisher: | NATURE PUBLISHING GROUP | Source: | SCIENTIFIC REPORTS, 6, p. 1-13 (Art N° 28115) | Abstract: | Bacteriophage therapy is currently resurging as a potential complement/alternative to antibiotic treatment. However, preclinical evaluation lacks streamlined approaches. We here focus on preclinical approaches which have been implemented to assess bacteriophage efficacy against Pseudomonas biofilms and infections. Laser interferometry and profilometry were applied to measure biofilm matrix permeability and surface geometry changes, respectively. These biophysical approaches were combined with an advanced Airway Surface Liquid infection model, which mimics in vitro the normal and CF lung environments, and an in vivo Galleria larvae model. These assays have been implemented to analyze KTN4 (279,593 bp dsDNA genome), a type-IV pili dependent, giant phage resembling phiKZ. Upon contact, KTN4 immediately disrupts the P. aeruginosa PAO1 biofilm and reduces pyocyanin and siderophore production. The gentamicin exclusion assay on NuLi-1 and CuFi-1 cell lines revealed the decrease of extracellular bacterial load between 4 and 7 logs and successfully prevents wild-type Pseudomonas internalization into CF epithelial cells. These properties and the significant rescue of Galleria larvae indicate that giant KTN4 phage is a suitable candidate for in vivo phage therapy evaluation for lung infection applications. | Notes: | [Danis-Wlodarczyk, Katarzyna; Olszak, Tomasz; Drulis-Kawa, Zuzanna] Univ Wroclaw, Inst Genet & Microbiol, Dept Pathogen Biol & Immunol, Wroclaw, Poland. [Danis-Wlodarczyk, Katarzyna; Vandenheuvel, Dieter; Bin Jang, Ho; Briers, Yves; Lavigne, Rob] KULeuven, Lab Gene Technol, Leuven, Belgium. [Briers, Yves] Univ Ghent, Dept Appl Biosci, Lab Appl Biotechnol, Ghent, Belgium. [Arabski, Michal] Jan Kochanowski Univ Humanities & Sci, Inst Biol, Dept Microbiol, Kielce, Poland. [Wasik, Slawomir] Jan Kochanowski Univ Humanities & Sci, Inst Phys, Dept Mol Phys, Kielce, Poland. [Drabik, Marcin] Jan Kochanowski Univ Humanities & Sci, Inst Phys, Dept Astrophys, Kielce, Poland. [Higgins, Gerard] Natl Children Res Ctr, Dublin, Ireland. [Higgins, Gerard; Tyrrell, Jean; Harvey, Brian J.] Beaumont Hosp, Royal Coll Surg Ireland, Dept Mol Med, Educ & Res Ctr, Dublin, Ireland. [Noben, Jean -Paul] Hasselt Univ, Biomed Res Inst, Sch Life Sci, Diepenbeek, Belgium. [Noben, Jean -Paul] Hasselt Univ, Transnat Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. | Document URI: | http://hdl.handle.net/1942/22717 | ISSN: | 2045-2322 | e-ISSN: | 2045-2322 | DOI: | 10.1038/srep28115 | ISI #: | 000384608500002 | Rights: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
danis 1.pdf | Published version | 1.37 MB | Adobe PDF | View/Open |
manuscript KTN4 19 02 2016 combined (1).pdf | Peer-reviewed author version | 3.37 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
31
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
71
checked on Oct 14, 2024
Page view(s)
70
checked on Sep 7, 2022
Download(s)
114
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.