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http://hdl.handle.net/1942/22761
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DC Field | Value | Language |
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dc.contributor.author | PLUSQUIN, Michelle | - |
dc.contributor.author | De Mulder, Katrien | - |
dc.contributor.author | VAN BELLEGHEM, Frank | - |
dc.contributor.author | DEGHESELLE, Olivier | - |
dc.contributor.author | PIROTTE, Nicky | - |
dc.contributor.author | WILLEMS, Maxime | - |
dc.contributor.author | CUYPERS, Ann | - |
dc.contributor.author | Salvenmoser, Willi | - |
dc.contributor.author | Ladurner, Peter | - |
dc.contributor.author | ARTOIS, Tom | - |
dc.contributor.author | SMEETS, Karen | - |
dc.date.accessioned | 2016-11-25T08:57:09Z | - |
dc.date.available | 2016-11-25T08:57:09Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | ENVIRONMENTAL TOXICOLOGY, 31(10), p. 1217-1228 | - |
dc.identifier.issn | 1520-4081 | - |
dc.identifier.uri | http://hdl.handle.net/1942/22761 | - |
dc.description.abstract | Stem cells or undifferentiated cells can cope more easily with external stresses. To evaluate the impact of toxic compounds on stem cell dynamics in vivo, in relation to other biological responses, we use the carcinogenic element cadmium and the regenerating model organism Macrostomum lignano. Through both BrdU and anti-histone H3 immunostainings, cadmium-induced effects were investigated at different stages of the stem cell cycle. A 24-h exposure to 100 and 250 M CdCl2 significantly decreased the number of stem cells (neoblasts) in mitosis, whereas the number of cells in the S phase remained unchanged. After this short-term exposure, the ultrastructure of the neoblasts was minimally affected in contrast to the epidermal tissues. These results were supported by gene expression data: transcripts of cdc2 and pig3 were significantly upregulated during all treatments. Both genes are involved in the cell cycle progression and are transcribed in the gonadal region, where stem cells are highly represented. Based on a substantial increase in gene expression of heat shock proteins (HSP) and their high activity in the gonadal region, we hypothesize that these proteins are key players in the protection of stem cells against external stresses. Apart from the strong HSP induction, other protective processes including cell division, apoptosis and anti-oxidative defence, were also activated. We, therefore, conclude that the protection of stem cells against external stressors may be based on the interplay between stem cell maintenance, i.e. repair and recovery through division, on one hand and apoptosis on the other hand. | - |
dc.description.sponsorship | Contract grant sponsors: Hasselt University BOF (Bijzonder OnderzoeksFonds) and Hasselt University tUL-impulsfinanciering. | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.rights | (c) 2015 Wiley Periodicals, Inc. | - |
dc.subject.other | toxicology; stem cell; macrostomum lignano; cadmium; stress | - |
dc.subject.other | toxicology; stem cell; Macrostomum lignano; cadmium; stress | - |
dc.title | Toxic effects of cadmium on flatworm stem cell dynamics: A transcriptomic and ultrastructural elucidation of underlying mechanisms | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 1228 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 1217 | - |
dc.identifier.volume | 31 | - |
local.format.pages | 12 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Plusquin, Michelle; Van Belleghem, Frank; DeGheselle, Olivier; Pirotte, Nicky; Cuypers, Ann; Artois, Tom; Smeets, Karen] Hasselt Univ, Dept Biol, Ctr Environm Sci, Agoralaan, Diepenbeek, Belgium. [De Mulder, Katrien; Salvenmoser, Willi; Ladurner, Peter] Univ Innsbruck, Inst Zool, Ctr Mol Biosci Innsbruck, Innsbruck, Austria. [Willems, Maxime] Univ Ghent, Pharmaceut Technol, St Pietersnieuwstr, Ghent, Belgium. [Van Belleghem, Frank] Open Univ, Fac Management Sci & Technol, Valkenburgerweg 177, Heerlen, Netherlands. | - |
local.publisher.place | HOBOKEN | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1002/tox.22129 | - |
dc.identifier.isi | 000383611900006 | - |
item.validation | ecoom 2017 | - |
item.contributor | PLUSQUIN, Michelle | - |
item.contributor | De Mulder, Katrien | - |
item.contributor | VAN BELLEGHEM, Frank | - |
item.contributor | DEGHESELLE, Olivier | - |
item.contributor | PIROTTE, Nicky | - |
item.contributor | WILLEMS, Maxime | - |
item.contributor | CUYPERS, Ann | - |
item.contributor | Salvenmoser, Willi | - |
item.contributor | Ladurner, Peter | - |
item.contributor | ARTOIS, Tom | - |
item.contributor | SMEETS, Karen | - |
item.accessRights | Restricted Access | - |
item.fullcitation | PLUSQUIN, Michelle; De Mulder, Katrien; VAN BELLEGHEM, Frank; DEGHESELLE, Olivier; PIROTTE, Nicky; WILLEMS, Maxime; CUYPERS, Ann; Salvenmoser, Willi; Ladurner, Peter; ARTOIS, Tom & SMEETS, Karen (2016) Toxic effects of cadmium on flatworm stem cell dynamics: A transcriptomic and ultrastructural elucidation of underlying mechanisms. In: ENVIRONMENTAL TOXICOLOGY, 31(10), p. 1217-1228. | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 1520-4081 | - |
crisitem.journal.eissn | 1522-7278 | - |
Appears in Collections: | Research publications |
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plusquin.pdf Restricted Access | Published version | 708.94 kB | Adobe PDF | View/Open Request a copy |
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