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http://hdl.handle.net/1942/23164
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DC Field | Value | Language |
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dc.contributor.author | GERVOIS, Pascal | - |
dc.contributor.author | WOLFS, Esther | - |
dc.contributor.author | DILLEN, Yorg | - |
dc.contributor.author | HILKENS, Petra | - |
dc.contributor.author | RATAJCZAK, Jessica | - |
dc.contributor.author | DRIESEN, Ronald | - |
dc.contributor.author | VANGANSEWINKEL, Tim | - |
dc.contributor.author | BRONCKAERS, Annelies | - |
dc.contributor.author | BRONE, Bert | - |
dc.contributor.author | STRUYS, Tom | - |
dc.contributor.author | LAMBRICHTS, Ivo | - |
dc.date.accessioned | 2017-02-22T08:31:14Z | - |
dc.date.available | 2017-02-22T08:31:14Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Journal of Dental Research, 96 (6), p. 654-662 | - |
dc.identifier.issn | 0022-0345 | - |
dc.identifier.uri | http://hdl.handle.net/1942/23164 | - |
dc.description.abstract | Neurological disorders are characterized by neurodegeneration and/or loss of neuronal function, which cannot be adequately repaired by the host. Therefore, there is need for novel treatment options such as cell-based therapies that aim to salvage or reconstitute the lost tissue or that stimulate host repair. The present study aimed to evaluate the paracrine effects of human dental pulp stem cells (hDPSCs) on the migration and neural maturation of human SH-SY5Y neuroblastoma cells. The hDPSC secretome had a significant chemoattractive effect on SH-SY5Y cells as shown by a transwell assay. To evaluate neural maturation, SH-SY5Y cells were first induced toward neuronal cells, after which they were exposed to the hDPSC secretome. In addition, SH-SY5Y cells subjected to the hDPSC secretome showed increased neuritogenesis compared with nonexposed cells. Maturated cells were shown to increase immune reactivity for neuronal markers compared with controls. Ultrastructurally, retinoic acid (RA) signaling and subsequent exposure to the hDPSC secretome induced a gradual rise in metabolic activity and neuronal features such as multivesicular bodies and cytoskeletal elements associated with cellular communication. In addition, electrophysiological recordings of differentiating cells demonstrated a transition toward a neuronal electrophysiological profile based on the maximum tetrodotoxin (TTX)–sensitive, Na+ current. Moreover, conditioned medium (CM)–hDPSC–maturated SH-SY5Y cells developed distinct features including, Cd2+-sensitive currents, which suggests that CM-hDPSC–maturated SH-SY5Y acquired voltage-gated Ca2+ channels. The results reported in this study demonstrate the potential of hDPSCs to support differentiation and recruitment of cells with neuronal precursor characteristics in a paracrine manner. Moreover, this in vitro experimental design showed that the widely used SH-SY5Y cell line can improve and simplify the preclinical in vitro research on the molecular mechanisms of stem cell–mediated neuronal regeneration. | - |
dc.description.sponsorship | We thank Dr. Wendy Martens for her help in the experimental design of the neuritogenesis assay. Furthermore, we thank Mr. Marc Jans and Mrs. Jeanine Santermans for their expertise in preparing TEM and immunocytochemical samples. We also thank Mrs. Petra Bex for her indispensable help with the electrophysiological recordings. E. Wolfs, J. Ratajczak, T. Vangansewinkel, P. Hilkens, Y. Dillen, and A. Bronckaers are funded by Fonds Wetenschappelijk Onderzoek by grants G0A7514N, G089213N, G029112N, 12D8516N, 1134717N, and 1508015N, respectively. Y. Dillen was also funded by Bijzonder Onderzoeksfonds grant BOF15DOC04 during part of this study. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article. | - |
dc.language.iso | en | - |
dc.rights | International & American Associations for Dental Research 2017 | - |
dc.subject.other | human dental pulp stem cells; SH-SY5Y neuroblastoma cells; paracrine-mediated maturation; migration assays; in vitro model; neuritogenesis | - |
dc.title | Paracrine Maturation and Migration of SH-SY5Y Cells by Dental Pulp Stem Cells | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 662 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 654 | - |
dc.identifier.volume | 96 | - |
local.format.pages | 9 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Lambrichts, I (reprint author), Hasselt Univ, Biomed Res Inst, Grp Morphol, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. ivo.lambrichts@uhasselt.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.class | dsPublValOverrule/author_version_not_expected | - |
dc.identifier.doi | 10.1177/0022034517690491 | - |
dc.identifier.isi | 000401914400008 | - |
item.contributor | GERVOIS, Pascal | - |
item.contributor | WOLFS, Esther | - |
item.contributor | DILLEN, Yorg | - |
item.contributor | HILKENS, Petra | - |
item.contributor | RATAJCZAK, Jessica | - |
item.contributor | DRIESEN, Ronald | - |
item.contributor | VANGANSEWINKEL, Tim | - |
item.contributor | BRONCKAERS, Annelies | - |
item.contributor | BRONE, Bert | - |
item.contributor | STRUYS, Tom | - |
item.contributor | LAMBRICHTS, Ivo | - |
item.fullcitation | GERVOIS, Pascal; WOLFS, Esther; DILLEN, Yorg; HILKENS, Petra; RATAJCZAK, Jessica; DRIESEN, Ronald; VANGANSEWINKEL, Tim; BRONCKAERS, Annelies; BRONE, Bert; STRUYS, Tom & LAMBRICHTS, Ivo (2017) Paracrine Maturation and Migration of SH-SY5Y Cells by Dental Pulp Stem Cells. In: Journal of Dental Research, 96 (6), p. 654-662. | - |
item.accessRights | Restricted Access | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2018 | - |
crisitem.journal.issn | 0022-0345 | - |
crisitem.journal.eissn | 1544-0591 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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Paracrine.pdf Restricted Access | Published version | 4.66 MB | Adobe PDF | View/Open Request a copy |
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