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Title: | Susceptibility of constructed wetland microbial communities to silver nanoparticles: A microcosm study | Authors: | Button, Mark Auvinen, Hannele Van Koetsem, Frederik HOSSEINKHANI, Baharak Rousseau, Diederik Weber, Kela P. Du Laing, Gijs |
Issue Date: | 2016 | Publisher: | ELSEVIER SCIENCE BV | Source: | ECOLOGICAL ENGINEERING, 97, p. 476-485 | Abstract: | Silver nanoparticles (AgNPs) are increasingly used as an antimicrobial agent in various consumer products. Silver release from these products occurs during use, washing and disposal at varying rates, into the wastewater system and eventually into aquatic ecosystems. Constructed wetlands (wetlands designed for water pollution control) represent a unique type of water treatment system which are beginning to receive growing amounts of influent AgNP loadings. In order to examine potential impacts to the health and ongoing utility of constructed wetlands we evaluated the susceptibility and the developed resistence of constructed wetland microbial communities to two types of AgNPs (citrate and polyvinylpyrrolidone (PVP)-coated), and ionic Ag (Ag+). Microcosms representing an unplanted batch-fed subsurface flow constructed wetland were exposed to an AgNP contaminated (100 mu g/l) simulated wastewater for 28 days. Experiments were triplicated and included unexposed controls. Analysis of total Ag in the interstitial water and biofilm matrix via inductively coupled plasma mass-spectrometry (ICP-MS) revealed the majority (>98%) of AgNPs partitioned from the interstitial water and accumulated in the biofilm and presumably the sediment matrix. No significant alteration to the microbial community function (assessed via community-level physiological profiling, CLPP) or structure (using denaturing gradient gel electrophoresis, DGGE) was observed following the initial 28 day exposure. Subsequent ex-situ dose-response testing over a wide concentration range (0-5 mu g/l) provided some evidence for increased resistance to Ag toxicity in the previously exposed microbial communities when compared to the controls. The ex-situ evaluated susceptibility of the microbial communities to AgNPs varied between treatments with significant reductions in catabolic activity observed at 0.5 mg/l for ionic Ag, 1 mg/l for citrate coated AgNPs and 5 mg/l for PVP coated AgNPs. These findings suggest that wetland microbial communities can tolerate and develop resistance to lower levels of in-situ AgNP exposure in a relatively short time, however ex-situ toxicity is observed past certain threshold concentrations and this threshold varies depending on the original form of the AgNP. (C) 2016 Elsevier B.V. All rights reserved. | Notes: | [Button, Mark; Weber, Kela P.] Royal Mil Coll Canada, Dept Chem & Chem Engn, Environm Sci Grp, POB 17000, Kingston, ON K7K 7B4, Canada. [Auvinen, Hannele; Van Koetsem, Frederik; Du Laing, Gijs] Univ Ghent, Fac Biosci Engn, Lab Analyt Chem & Appl Ecochem, Coupure Links 653, B-9000 Ghent, Belgium. [Auvinen, Hannele; Rousseau, Diederik] Univ Ghent, Fac Biosci Engn, Lab Ind Water & Ecotechnol, Graaf Karel de Goedelaan 5, B-8500 Kortrijk, Belgium. [Hosseinkhani, Baharak] Univ Ghent, Fac Biosci Engn, Ctr Microbial Ecol & Technol, Coupure Links 653, B-9000 Ghent, Belgium. [Hosseinkhani, Baharak] Hasselt Univ, Biomed Res Inst, Martelarenlaan 42, B-3500 Hasselt, Belgium. | Keywords: | constructed wetland; microbial community; biofilm; silver nanoparticle; resistance; toxicity; community level physiological profiling; denaturing gradient gel electrophoresis;Constructed wetland; Microbial community; Biofilm; Silver nanoparticle; Resistance; Toxicity; Community level physiological profiling; Denaturing gradient gel electrophoresis | Document URI: | http://hdl.handle.net/1942/23225 | ISSN: | 0925-8574 | e-ISSN: | 1872-6992 | DOI: | 10.1016/j.ecoleng.2016.10.033 | ISI #: | 000388580200050 | Rights: | © 2016 Elsevier B.V. All rights reserved | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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button 1.pdf Restricted Access | Published version | 1.6 MB | Adobe PDF | View/Open Request a copy |
Button et al EE July 20 2016.pdf | Peer-reviewed author version | 597.02 kB | Adobe PDF | View/Open |
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