Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/23249
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Le Blon, Debbie | - |
dc.contributor.author | Guglielmetti, Caroline | - |
dc.contributor.author | Hoornaert, Chloe | - |
dc.contributor.author | Quarta, Alessandra | - |
dc.contributor.author | Daans, Jasmijn | - |
dc.contributor.author | DOOLEY, Dearbhaile | - |
dc.contributor.author | LEMMENS, Evi | - |
dc.contributor.author | Praet, Jelle | - |
dc.contributor.author | De Vocht, Nathalie | - |
dc.contributor.author | Reekmans, Kristien | - |
dc.contributor.author | SANTERMANS, Eva | - |
dc.contributor.author | HENS, Niel | - |
dc.contributor.author | Goossens, Herman | - |
dc.contributor.author | Verhoye, Marleen | - |
dc.contributor.author | Van der Linden, Annemie | - |
dc.contributor.author | Berneman, Zwi | - |
dc.contributor.author | HENDRIX, Sven | - |
dc.contributor.author | Ponsaerts, Peter | - |
dc.date.accessioned | 2017-02-28T10:00:00Z | - |
dc.date.available | 2017-02-28T10:00:00Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | JOURNAL OF NEUROINFLAMMATION, 13 (Art N° 288) | - |
dc.identifier.issn | 1742-2094 | - |
dc.identifier.uri | http://hdl.handle.net/1942/23249 | - |
dc.description.abstract | Background: Promoting the neuroprotective and repair-inducing effector functions of microglia and macrophages, by means of M2 polarisation or alternative activation, is expected to become a new therapeutic approach for central nervous system (CNS) disorders in which detrimental pro-inflammatory microglia and/or macrophages display a major contribution to the neuropathology. In this study, we present a novel in vivo approach using intracerebral grafting of mesenchymal stem cells (MSC) genetically engineered to secrete interleukin 13 (IL13-MSC). Methods: In the first experimental setup, control MSC and IL13-MSC were grafted in the CNS of eGFP(+) bone marrow chimaeric C57BL/6 mice to histologically evaluate IL13-mediated expression of several markers associated with alternative activation, including arginase1 and Ym1, on MSC graft-recognising microglia and MSC graft-infiltrating macrophages. In the second experimental setup, IL13-MSC were grafted on the right side (or on both the right and left sides) of the splenium of the corpus callosum in wild-type C57BL/6 mice and in C57BL/6 CX(3)CR1(eGFP/+)CCR2(RFP/+) transgenic mice. Next, CNS inflammation and demyelination was induced by means of a cuprizone-supplemented diet. The influence of IL13-MSC grafting on neuropathological alterations was monitored by non-invasive T2-weighted magnetic resonance imaging (MRI) and quantitative histological analyses, as compared to cuprizone-treated mice with control MSC grafts and/or cuprizone-treated mice without MSC injection. Results: In the first part of this study, we demonstrate that MSC graft-associated microglia and MSC graft-infiltrating macrophages are forced into alternative activation upon grafting of IL13-MSC, but not upon grafting of control MSC. In the second part of this study, we demonstrate that grafting of IL13-MSC, in addition to the recruitment of M2 polarised macrophages, limits cuprizone-induced microgliosis, oligodendrocyte death and demyelination. Furthermore, we here demonstrate that injection of IL13-MSC at both sides of the splenium leads to a superior protective effect as compared to a single injection at one side of the splenium. Conclusions: Controlled and localised production of IL13 by means of intracerebral MSC grafting has the potential to modulate cell graft-and pathology-associated microglial/macrophage responses, and to interfere with oligodendrocyte death and demyelinating events in the cuprizone mouse model. | - |
dc.description.sponsorship | This work was supported by research grants G.0136.11 (granted to PP, AVDL and ZB), G.0130.11 (granted to PP, AVDL and ZB) and G.0834.11 (granted to PP and SH) of the Fund for Scientific Research-Flanders (FWO-Vlaanderen, Belgium), in part by a Methusalem research grant from the Flemish government (granted to HG), in part by funding received from the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement 278850 (INMiND) (granted to AVdL) and in part by funding received from the Belgian Charcot Foundation (granted to PP and AVDL) for the design of the study; collection, analysis and interpretation of data; and writing the manuscript. | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.rights | © The Author(s). 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | - |
dc.subject.other | Interleukin 13; Mesenchymal stem cells; Neuroinflammation; Transplantation; Magnetic resonance imaging | - |
dc.subject.other | interleukin 13; mesenchymal stem cells; neuroinflammation; transplantation; magnetic resonance imaging | - |
dc.title | Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model | - |
dc.type | Journal Contribution | - |
dc.identifier.volume | 13 | - |
local.format.pages | 20 | - |
local.format.pages | 20 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Le Blon, Debbie; Hoornaert, Chloe; Quarta, Alessandra; Daans, Jasmijn; De Vocht, Nathalie; Reekmans, Kristien; Berneman, Zwi; Ponsaerts, Peter] Univ Antwerp, Lab Expt Hematol, Univ Pl 1, B-2610 Antwerp, Belgium. [Le Blon, Debbie; Hoornaert, Chloe; Quarta, Alessandra; Daans, Jasmijn; De Vocht, Nathalie; Reekmans, Kristien; Goossens, Herman; Berneman, Zwi; Ponsaerts, Peter] Univ Antwerp, Vaccine & Infect Dis Inst, Univ Pl 1, B-2610 Antwerp, Belgium. [Guglielmetti, Caroline; Praet, Jelle; Verhoye, Marleen; Van der Linden, Annemie] Univ Antwerp, Bioimaging Lab, Univ Pl 1, B-2610 Antwerp, Belgium. [Dooley, Dearbhaile; Lemmens, Evi; Hendrix, Sven] Hasselt Univ, Biomed Res Inst, Dept Morphol, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Santermans, Eva; Hens, Niel] Hasselt Univ, I Biostat, Ctr Stat, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Hens, Niel] Univ Antwerp, Chermid, Univ Pl 1, B-2610 Antwerp, Belgium. [Ponsaerts, Peter] Univ Antwerp, Vaccine & Infect Dis Inst Vaxinfectio, Lab Expt Hematol, Expt Cell Transplantat Grp, Campus Drie Eiken CDE S6-51,Univ Pl 1, B-2610 Antwerp, Belgium. | - |
local.publisher.place | LONDON | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 288 | - |
local.class | dsPublValOverrule/author_version_not_expected | - |
dc.identifier.doi | 10.1186/s12974-016-0756-7 | - |
dc.identifier.isi | 000390713400002 | - |
item.contributor | Le Blon, Debbie | - |
item.contributor | Guglielmetti, Caroline | - |
item.contributor | Hoornaert, Chloe | - |
item.contributor | Quarta, Alessandra | - |
item.contributor | Daans, Jasmijn | - |
item.contributor | DOOLEY, Dearbhaile | - |
item.contributor | LEMMENS, Evi | - |
item.contributor | Praet, Jelle | - |
item.contributor | De Vocht, Nathalie | - |
item.contributor | Reekmans, Kristien | - |
item.contributor | SANTERMANS, Eva | - |
item.contributor | HENS, Niel | - |
item.contributor | Goossens, Herman | - |
item.contributor | Verhoye, Marleen | - |
item.contributor | Van der Linden, Annemie | - |
item.contributor | Berneman, Zwi | - |
item.contributor | HENDRIX, Sven | - |
item.contributor | Ponsaerts, Peter | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2018 | - |
item.fullcitation | Le Blon, Debbie; Guglielmetti, Caroline; Hoornaert, Chloe; Quarta, Alessandra; Daans, Jasmijn; DOOLEY, Dearbhaile; LEMMENS, Evi; Praet, Jelle; De Vocht, Nathalie; Reekmans, Kristien; SANTERMANS, Eva; HENS, Niel; Goossens, Herman; Verhoye, Marleen; Van der Linden, Annemie; Berneman, Zwi; HENDRIX, Sven & Ponsaerts, Peter (2016) Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model. In: JOURNAL OF NEUROINFLAMMATION, 13 (Art N° 288). | - |
item.accessRights | Open Access | - |
crisitem.journal.eissn | 1742-2094 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
le blon 1.pdf | Published version | 3.43 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
14
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
33
checked on Apr 30, 2024
Page view(s)
70
checked on Sep 7, 2022
Download(s)
118
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.