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Title: | Arabidopsis plants exposed to gamma radiation in two successive generations show a different oxidative stress response | Authors: | VAN DE WALLE, Jorden HOREMANS, Nele SAENEN, Eline Van Hees, May Wannijn, Jean Nauts, Robin van Gompel, Axel VANGRONSVELD, Jaco VANDENHOVE, Hildegarde CUYPERS, Ann |
Issue Date: | 2016 | Publisher: | ELSEVIER SCI LTD | Source: | JOURNAL OF ENVIRONMENTAL RADIOACTIVITY, 165, p. 270-279 | Abstract: | When terrestrial environments get contaminated with long-lived gamma emitting radionuclides, plants that grow in these contaminated areas are exposed to gamma radiation during consecutive generations. Therefore it is important to evaluate the gamma induced stress response in plants in and between generations. The objective of this research is to reveal differences at the level of the antioxidative stress response between generations with a different radiation history. An experiment was conducted in which 7-days old Arabidopsis thaliana plants were exposed for 14 days to four different gamma dose rates: 22 mGy/h, 38 mGy/h, 86 mGy/h and 457 mGy/h. Two different plant groups were used: plants that were not exposed to gamma radiation before (P0) and plants that received the aforementioned gamma treatment during their previous generation (S1). Growth, the concentration of the antioxidants ascorbate and glutathione, a number of antioxidative enzyme activities and their gene transcript levels were analysed. A dose-rate dependent induction was seen for catalase (CAT) and guaiacol peroxidase (GPX) in the roots and for syringaldazine peroxidase (SPX) in the shoots. Differences between the two generations were observed for CAT and GPX in the roots, where a significantly higher activity of these reactive oxygen species (ROS) detoxifying enzymes was observed in the S1 generation. For SPX in the shoots, a dose dependent upregulation was observed in the PO generation. However, high SPX activities were present for all doses in the S1 generation. These differences in enzyme activity between generations for SPX and GPX and the involvement of these enzymes in cell wall biosynthesis, suggest an important role for cell wall strengthening in the response to gamma irradiation. (C) 2016 Elsevier Ltd. All rights reserved. | Notes: | [van de Walle, Jorden; Horemans, Nele; Saenen, Eline; Van Hees, May; Wannijn, Jean; Nauts, Robin; van Gompel, Axel; Vandenhove, Hildegarde] SCK CEN, Belgian Nucl Res Ctr, Mol, Belgium. [van de Walle, Jorden; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium. | Keywords: | gamma radiation; arabidopsis thaliana; oxidative stress; generations; cell wall strengthening;Gamma radiation; Arabidopsis thaliana; Oxidative stress; Generations; Cell wall strengthening | Document URI: | http://hdl.handle.net/1942/23250 | ISSN: | 0265-931X | e-ISSN: | 1879-1700 | DOI: | 10.1016/j.jenvrad.2016.10.014 | ISI #: | 000390500100030 | Rights: | © 2016 Elsevier Ltd. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2018 |
Appears in Collections: | Research publications |
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van de walle 1.pdf Restricted Access | Published version | 1.37 MB | Adobe PDF | View/Open Request a copy |
Arabidopsis.pdf | Peer-reviewed author version | 641 kB | Adobe PDF | View/Open |
Fig1.jpg | Supplementary material | 111.67 kB | JPEG | View/Open |
Fig2.jpg | Supplementary material | 46.34 kB | JPEG | View/Open |
Fig3A.jpg | Supplementary material | 17.53 kB | JPEG | View/Open |
Fig3B.jpg | Supplementary material | 16.47 kB | JPEG | View/Open |
Fig4.jpg | Supplementary material | 28.9 kB | JPEG | View/Open |
Fig5.jpg | Supplementary material | 61.8 kB | JPEG | View/Open |
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