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Title: | Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines | Authors: | Wang, P Keijer, J Bouwman, F MARIMAN, Edwin NOBEN, Jean-Paul ROBBEN, Johan RENES, J. |
Issue Date: | 2004 | Publisher: | BIRKHAUSER VERLAG AG | Source: | CELLULAR AND MOLECULAR LIFE SCIENCES, 61(18). p. 2405-2417 | Abstract: | Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20degreesC treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation. | Notes: | Maastricht Univ, Dept Human Biol, Nutr & Toxicol Res Inst Maastricht, Maastricht Prote Ctr, NL-6200 MD Maastricht, Netherlands. RIKILT Inst Food Safety, NL-6700 AE Wageningen, Netherlands. Limburgs Univ Ctr, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Transnatl Univ Limburg, Sch Life Sci, B-3590 Diepenbeek, Belgium.Renes, J, Maastricht Univ, Dept Human Biol, Nutr & Toxicol Res Inst Maastricht, Maastricht Prote Ctr, POB 616, NL-6200 MD Maastricht, Netherlands.J.Renes@HB.unimaas.nl | Keywords: | 3T3-L1 adipocyte; secretion; proteomics; extracellular matrix; growth regulation | Document URI: | http://hdl.handle.net/1942/2363 | ISSN: | 1420-682X | e-ISSN: | 1420-9071 | DOI: | 10.1007/s00018-004-4256-z | ISI #: | 000224525900010 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2005 |
Appears in Collections: | Research publications |
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