Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/23717
Title: | Scavenger receptor collectin placenta 1 is a novel receptor 1 involved in the uptake of myelin by phagocytes | Authors: | BOGIE, Jeroen MAILLEUX, Jo WOUTERS, Elien JORISSEN, Winde GRAJCHEN, Elien VANMOL, Jasmine WOUTERS, Kristiaan HELLINGS, Niels Van Horsen, Jack VANMIERLO, Tim HENDRIKS, Jerome |
Issue Date: | 2017 | Source: | Scientific Reports, 7, p. 1-9 (Art N° 44794) | Abstract: | Myelin-containing macrophages and microglia are the most abundant immune cells in active multiple sclerosis (MS) lesions. Our recent transcriptomic analysis demonstrated that collectin placenta 1 (CL-P1) is one of the most potently induced genes in macrophages after uptake of myelin. CL-P1 is a type II transmembrane protein with both a collagen-like and carbohydrate recognition domain, which plays a key role in host defense. In this study we sought to determine the dynamics of CL-P1 expression on myelin-containing phagocytes and define the role that it plays in MS lesion development. We show that myelin uptake increases the cell surface expression of CL-P1 by mouse and human macrophages, but not by primary mouse microglia in vitro. In active demyelinating MS lesions, CL-P1 immunoreactivity was localized to perivascular and parenchymal myelin-laden phagocytes. Finally, we demonstrate that CL-P1 is involved in myelin internalization as knockdown of CL-P1 markedly reduced myelin uptake. Collectively, our data indicate that CL-P1 is a novel receptor involved in myelin uptake by phagocytes and likely plays a role in MS lesion development. | Notes: | Hendriks, JJA (reprint author) , Hasselt Univ, Transnat Univ Limburg, Sch Life Sci, Inst Biomed Res, Diepenbeek, Belgium. Jerome.hendriks@uhasselt.be | Document URI: | http://hdl.handle.net/1942/23717 | ISSN: | 2045-2322 | e-ISSN: | 2045-2322 | DOI: | 10.1038/srep44794 | ISI #: | 000397177700001 | Rights: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2018 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
CL-P1 auteursversie.pdf | Peer-reviewed author version | 816.25 kB | Adobe PDF | View/Open |
srep44794.pdf | Published version | 1.4 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.