Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/23760
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dc.contributor.authorWiens, Matthew D.-
dc.contributor.authorShen, Yi-
dc.contributor.authorLi, Xi-
dc.contributor.authorSalem, M. Alaraby-
dc.contributor.authorSMISDOM, Nick-
dc.contributor.authorZhang, Wei-
dc.contributor.authorBrown, Alex-
dc.contributor.authorCampbell, Robert E.-
dc.date.accessioned2017-05-18T13:27:36Z-
dc.date.available2017-05-18T13:27:36Z-
dc.date.issued2016-
dc.identifier.citationCHEMBIOCHEM, 17(24), p. 2361-2367-
dc.identifier.issn1439-4227-
dc.identifier.urihttp://hdl.handle.net/1942/23760-
dc.description.abstractThe tetrameric red fluorescent protein from Discosoma sp. coral (DsRed) has previously been engineered to produce dimeric and monomeric fluorescent variants with excitation and emission profiles that span the visible spectrum. The brightest of the effectively monomeric DsRed variants is tdTomato-a tandem fusion of a dimeric DsRed variant. Here we describe the engineering of brighter red (RRvT), green (GGvT), and green-red heterodimeric (GRvT) tdTomato variants. GRvT exhibited 99% intramolecular FRET efficiency, resulting in long Stokes shift red fluorescence. These new variants could prove useful for multicolor live-cell imaging applications.-
dc.description.sponsorshipWe thank the University of Alberta Molecular Biology Services Unit (MBSU) for DNA sequencing and technical assistance, along with Ahmed Abdelfattah, Klaus Ballanyi, and Nils Petersen for assistance with imaging and smFRET measurements. This work was supported by grants from the Canadian Institutes for Health Research (CIHR; MOP-123514) to R.E.C. and the Natural Sciences and Engineering Research Council of Canada (NSERC; RGPIN 288338-2010) to R.E.C. and (RGPIN-2015-05341) to A.B. M.D.W. was supported in part by a Queen Elizabeth II scholarship from the University of Alberta. Y.S. was supported by an Alberta Innovates scholarship.-
dc.language.isoen-
dc.publisherWILEY-V C H VERLAG GMBH-
dc.rights(C) 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim-
dc.subject.otherdimerization; directed evolution; fluorescence; FRET; protein engineering-
dc.subject.otherdimerization; directed evolution; fluorescence; FRET; protein engineering-
dc.titleA Tandem Green-Red Heterodimeric Fluorescent Protein with High FRET Efficiency-
dc.typeJournal Contribution-
dc.identifier.epage2367-
dc.identifier.issue24-
dc.identifier.spage2361-
dc.identifier.volume17-
local.format.pages7-
local.bibliographicCitation.jcatA1-
dc.description.notes[Wiens, Matthew D.; Shen, Yi; Li, Xi; Salem, M. Alaraby; Smisdom, Nick; Zhang, Wei; Brown, Alex; Campbell, Robert E.] Univ Alberta, Dept Chem, 11227 Saskatchewan Dr, Edmonton, AB T6G 2G2, Canada. [Smisdom, Nick] Univ Hasselt, Fac Med & Life Sci, Martelarenlaan 42, B-3500 Hasselt, Belgium.-
local.publisher.placeWEINHEIM-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1002/cbic.201600492-
dc.identifier.isi000392934100012-
item.validationecoom 2018-
item.contributorWiens, Matthew D.-
item.contributorShen, Yi-
item.contributorLi, Xi-
item.contributorSalem, M. Alaraby-
item.contributorSMISDOM, Nick-
item.contributorZhang, Wei-
item.contributorBrown, Alex-
item.contributorCampbell, Robert E.-
item.accessRightsRestricted Access-
item.fullcitationWiens, Matthew D.; Shen, Yi; Li, Xi; Salem, M. Alaraby; SMISDOM, Nick; Zhang, Wei; Brown, Alex & Campbell, Robert E. (2016) A Tandem Green-Red Heterodimeric Fluorescent Protein with High FRET Efficiency. In: CHEMBIOCHEM, 17(24), p. 2361-2367.-
item.fulltextWith Fulltext-
crisitem.journal.issn1439-4227-
crisitem.journal.eissn1439-7633-
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