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http://hdl.handle.net/1942/23770
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DC Field | Value | Language |
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dc.contributor.author | Jorge-Peñas, Alvaro | - |
dc.contributor.author | BOVE, Hannelore | - |
dc.contributor.author | SANEN, Kathleen | - |
dc.contributor.author | Vaeyens, Marie-Mo | - |
dc.contributor.author | Steuwe, Christian | - |
dc.contributor.author | Roeffaers, Maarten | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.contributor.author | Van Oosterwyck, Hans | - |
dc.date.accessioned | 2017-05-22T12:06:56Z | - |
dc.date.available | 2017-05-22T12:06:56Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Biomaterials, 136, pag. 86-97 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | http://hdl.handle.net/1942/23770 | - |
dc.description.abstract | To advance our current understanding of cell-matrix mechanics and its importance for biomaterials development, advanced three-dimensional (3D) measurement techniques are necessary. Cell-induced deformations of the surrounding matrix are commonly derived from the displacement of embedded fiducial markers, as part of traction force microscopy (TFM) procedures. However, these fluorescent markers may alter the mechanical properties of the matrix or can be taken up by the embedded cells, and therefore influence cellular behavior and fate. In addition, the currently developed methods for calculating cell-induced deformations are generally limited to relatively small deformations, with displacement magnitudes and strains typically of the order of a few microns and less than 10% respectively. Yet, large, complex deformation fields can be expected from cells exerting tractions in fibrillar biomaterials, like collagen. To circumvent these hurdles, we present a technique for the 3D full-field quantification of large cell-generated deformations in collagen, without the need of fiducial markers. We applied non-rigid, Free Form Deformation (FFD)-based image registration to compute full-field displacements induced by MRC-5 human lung fibroblasts in a collagen type I hydrogel by solely relying on second harmonic generation (SHG) from the collagen fibrils. By executing comparative experiments, we show that comparable displacement fields can be derived from both fibrils and fluorescent beads. SHG-based fibril imaging can circumvent all described disadvantages of using fiducial markers. This approach allows measuring 3D full-field deformations under large displacement (of the order of 10 µm) and strain regimes (up to 40%). As such, it holds great promise for the study of large cell-induced deformations as an inherent component of cell-biomaterial interactions and cell-mediated biomaterial remodeling. | - |
dc.description.sponsorship | H.B. acknowledges funding from Research Foundation Flanders (Fonds Wetenschappelijk Onderzoek, FWO) for a doctoral fellowship: 11ZB115N. C.S. acknowledges funding from Research Foundation Flanders (Fonds Wetenschappelijk Onderzoek, FWO) for a postdoctoral fellowship: 12R6315N. The authors also thank FWO for the research grants G0821.13 and G.0B96.15. H.V.O. acknowledges that the research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/(ERC Grant Agreement no 308223). M.A. thanks the Province of Limburg (Belgium) for the financial support within the tUL IMPULS FASE II program, allowing for the upgrading of the laser source used in this work. Prof. M. vandeVen is acknowledged for his theoretical support. H.B. gratefully acknowledges the assistance of Mrs. P. Bex. | - |
dc.language.iso | en | - |
dc.subject.other | large deformation; fibrillar hydrogel; second Harmonic Generation; traction force microscopy; non-rigid image registration | - |
dc.title | 3D full-field quantification of cell-induced large deformations in fibrillar biomaterials by combining non-rigid image registration with label-free second harmonic generation | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 97 | - |
dc.identifier.spage | 86 | - |
dc.identifier.volume | 136 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Van Oosterwyck, H (reprint author), Katholieke Univ Leuven, Dept Mech Engn, Celestijnenlaan 300C Box 2419, Leuven, Belgium. marcel.ameloot@uhasselt.be; hans.vanoosterwyck@kuleuven.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.status | In Press | - |
dc.identifier.doi | 10.1016/j.biomaterials.2017.05.015 | - |
dc.identifier.isi | 000401816500007 | - |
item.fulltext | With Fulltext | - |
item.contributor | Jorge-Peñas, Alvaro | - |
item.contributor | BOVE, Hannelore | - |
item.contributor | SANEN, Kathleen | - |
item.contributor | Vaeyens, Marie-Mo | - |
item.contributor | Steuwe, Christian | - |
item.contributor | Roeffaers, Maarten | - |
item.contributor | AMELOOT, Marcel | - |
item.contributor | Van Oosterwyck, Hans | - |
item.accessRights | Closed Access | - |
item.validation | ecoom 2018 | - |
item.fullcitation | Jorge-Peñas, Alvaro; BOVE, Hannelore; SANEN, Kathleen; Vaeyens, Marie-Mo; Steuwe, Christian; Roeffaers, Maarten; AMELOOT, Marcel & Van Oosterwyck, Hans (2017) 3D full-field quantification of cell-induced large deformations in fibrillar biomaterials by combining non-rigid image registration with label-free second harmonic generation. In: Biomaterials, 136, pag. 86-97. | - |
crisitem.journal.issn | 0142-9612 | - |
crisitem.journal.eissn | 1878-5905 | - |
Appears in Collections: | Research publications |
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