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Title: | Maternal intake of methyl-group donors affects DNA methylation of metabolic genes in infants | Authors: | Pauwels, Sara Ghosh, Manosij Duca, Radu Corneliu Bekaert, Bram Freson, Kathleen Huybrechts, Inge LANGIE, Sabine Koppen, Gudrun Devlieger, Roland Godderis, Lode |
Issue Date: | 2017 | Publisher: | BIOMED CENTRAL LTD | Source: | CLINICAL EPIGENETICS, 9, p. 1-13 (Art N° 16) | Abstract: | Background: Maternal nutrition during pregnancy and infant nutrition in the early postnatal period (lactation) are critically involved in the development and health of the newborn infant. The Maternal Nutrition and Offspring's Epigenome (MANOE) study was set up to assess the effect of maternal methyl-group donor intake (choline, betaine, folate, methionine) on infant DNA methylation. Maternal intake of dietary methyl-group donors was assessed using a food-frequency questionnaire (FFQ). Before and during pregnancy, we evaluated maternal methyl-group donor intake through diet and supplementation (folic acid) in relation to gene-specific (IGF2 DMR, DNMT1, LEP, RXRA) buccal epithelial cell DNA methylation in 6 months old infants (n = 114) via pyrosequencing. In the early postnatal period, we determined the effect of maternal choline intake during lactation (in mothers who breast-fed for at least 3 months) on gene-specific buccal DNA methylation (n = 65). Results: Maternal dietary and supplemental intake of methyl-group donors (folate, betaine, folic acid), only in the periconception period, was associated with buccal cell DNA methylation in genes related to growth (IGF2 DMR), metabolism (RXRA), and appetite control (LEP). A negative association was found between maternal folate and folic acid intake before pregnancy and infant LEP (slope = -1.233, 95% CI -2.342; -0.125, p = 0.0298) and IGF2 DMR methylation (slope = -0.706, 95% CI -1.242; -0.107, p = 0.0101), respectively. Positive associations were observed for maternal betaine (slope = 0.875, 95% CI 0.118; 1.633, p = 0.0241) and folate (slope = 0.685, 95% CI 0.245; 1.125, p = 0.0027) intake before pregnancy and RXRA methylation. Buccal DNMT1 methylation in the infant was negatively associated with maternal methyl-group donor intake in the first and second trimester of pregnancy and negatively in the third trimester. We found no clear association between maternal choline intake during lactation and buccal infant DNA methylation. Conclusions: This study suggests that maternal dietary and supplemental intake of methyl-group donors, especially in the periconception period, can influence infant's buccal DNA methylation in genes related to metabolism, growth, appetite regulation, and maintenance of DNA methylation reactions. | Notes: | [Pauwels, Sara; Ghosh, Manosij; Duca, Radu Corneliu; Godderis, Lode] Univ Leuven, KU Leuven, Dept Publ Hlth & Primary Care, Environm & Hlth, Kapucijnenvoer 35 Blok D Box 7001, B-3000 Leuven, Belgium. [Pauwels, Sara; Langie, Sabine A. S.; Koppen, Gudrun] Flemish Inst Technol Res VITO, Unit Environm Risk & Hlth, Boeretang 200, B-2400 Mol, Belgium. [Bekaert, Bram] Univ Leuven, KU Leuven, Dept Imaging & Pathol, B-3000 Leuven, Belgium. [Bekaert, Bram] Univ Leuven, KU Leuven, Univ Hosp Leuven, B-3000 Leuven, Belgium. [Bekaert, Bram] Univ Leuven, KU Leuven, Dept Forens Med, B-3000 Leuven, Belgium. [Bekaert, Bram] Univ Leuven, KU Leuven, Lab Forens Genet & Mol Archeol, B-3000 Leuven, Belgium. [Freson, Kathleen] Univ Leuven, KU Leuven, Ctr Mol & Vasc Biol, UZ Herestr 49,Box 911, B-3000 Leuven, Belgium. [Huybrechts, Inge] Int Agcy Res Canc, 150 Cours Albert Thomas, F-69372 Lyon 08, France. [Langie, Sabine A. S.] Hasselt Univ, Fac Sci, B-3590 Diepenbeek, Belgium. [Devlieger, Roland] Univ Leuven, KU Leuven, Dept Dev & Regenerat, B-3000 Leuven, Belgium. [Devlieger, Roland] Univ Hosp Leuven, Dept Obstet & Gynecol, B-3000 Leuven, Belgium. [Godderis, Lode] IDEWE, External Serv Prevent & Protect Work, Interleuvenlaan 58, B-3001 Heverlee, Belgium. | Keywords: | methyl-group donors; DNA methylation; LEP; IGF2 DMR; RXRA; DNMT1; lactation; pregnancy;Methyl-group donors; DNA methylation; LEP; IGF2 DMR; RXRA; DNMT1; Lactation; Pregnancy | Document URI: | http://hdl.handle.net/1942/23935 | ISSN: | 1868-7075 | e-ISSN: | 1868-7083 | DOI: | 10.1186/s13148-017-0321-y | ISI #: | 000393925300002 | Rights: | © The Author(s). 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2018 |
Appears in Collections: | Research publications |
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