Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/24119
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mesa, Victoria | - |
dc.contributor.author | NAVAZAS, Alejandro | - |
dc.contributor.author | Gonzalez-Gil, Ricardo | - |
dc.contributor.author | Gonzalez, Aida | - |
dc.contributor.author | WEYENS, Nele | - |
dc.contributor.author | Lauga, Beatrice | - |
dc.contributor.author | Gallego, Jose Luis R. | - |
dc.contributor.author | Sanchez, Jesus | - |
dc.contributor.author | Isabel Pelaez, Ana | - |
dc.date.accessioned | 2017-08-07T10:45:56Z | - |
dc.date.available | 2017-08-07T10:45:56Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(8), p. e03411-e03416 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/1942/24119 | - |
dc.description.abstract | The aim of this study was to investigate the potential of indigenous arsenic-tolerant bacteria to enhance arsenic phytoremediation by the autochthonous pseudometallophyte Betula celtiberica. The first goal was to perform an initial analysis of the entire rhizosphere and endophytic bacterial communities of the above-named accumulator plant, including the cultivable bacterial species. B. celtiberica's microbiome was dominated by taxa related to Flavobacteriales, Burkholderiales, and Pseudomonadales, especially the Pseudomonas and Flavobacterium genera. A total of 54 cultivable rhizobacteria and 41 root endophytes, mainly affiliated with the phyla Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria, were isolated and characterized with respect to several potentially useful features for metal plant accumulation, such as the ability to promote plant growth, metal chelation, and/or mitigation of heavy-metal stress. Seven bacterial isolates were further selected and tested for in vitro accumulation of arsenic in plants; four of them were finally assayed in field-scale bioaugmentation experiments. The exposure to arsenic in vitro caused an increase in the total nonprotein thiol compound content in roots, suggesting a detoxification mechanism through phytochelatin complexation. In the contaminated field, the siderophore and indole-3-acetic acid producers of the endophytic bacterial consortium enhanced arsenic accumulation in the leaves and roots of Betula celtiberica, whereas the rhizosphere isolate Ensifer adhaerens strain 91R mainly promoted plant growth. Field experimentation showed that additional factors, such as soil arsenic content and pH, influenced arsenic uptake in the plant, attesting to the relevance of field conditions in the success of phytoextraction strategies. IMPORTANCE Microorganisms and plants have developed several ways of dealing with arsenic, allowing them to resist and metabolize this metalloid. These properties form the basis of phytoremediation treatments and the understanding that the interactions of plants with soil bacteria are crucial for the optimization of arsenic uptake. To address this in our work, we initially performed a microbiome analysis of the autochthonous Betula celtiberica plants growing in arsenic-contaminated soils, including endosphere and rhizosphere bacterial communities. We then proceeded to isolate and characterize the cultivable bacteria that were potentially better suited to enhance phytoextraction efficiency. Eventually, we went to the field application stage. Our results corroborated the idea that recovery of pseudometallophyte-associated bacteria adapted to a large historically contaminated site and their use in bioaugmentation technologies are affordable experimental approaches and potentially very useful for implementing effective phytoremediation strategies with plants and their indigenous bacteria. | - |
dc.description.sponsorship | This research was supported by the LIFE I + DARTS (LIFE11 ENV/ES/000547) and UHasselt Methusalem project 08M03VGRJ. V. Mesa was supported by the Francisco Jose de Caldas Scholarship Program (Administrative Department of Science, Technology and Innovation, COLCIENCIAS). A. I. Pelaez was supported by grant FC-15-GRUPIN14-107 from the Consejeria de Economia y Empleo del Principado de Asturias, Spain. | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.rights | Copyright © 2017 American Society for Microbiology. All Rights Reserved. | - |
dc.subject.other | contaminated soil; arsenic; root endophytes; rhizobacteria; Betula; field-scale study; phytoextraction; bioaugmentation | - |
dc.subject.other | contaminated soil; arsenic; root endophytes; rhizobacteria; Betula; field-scale study; phytoextraction; bioaugmentation | - |
dc.title | Use of Endophytic and Rhizosphere Bacteria To Improve Phytoremediation of Arsenic-Contaminated Industrial Soils by Autochthonous Betula celtiberica | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | e03416 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | e03411 | - |
dc.identifier.volume | 83 | - |
local.format.pages | 18 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Mesa, Victoria; Sanchez, Jesus; Isabel Pelaez, Ana] Univ Oviedo, Dept Biol Func IUBA, Oviedo, Spain. [Navazas, Alejandro; Gonzalez-Gil, Ricardo; Gonzalez, Aida] Univ Oviedo, Dept Biol Organismos & Sistemas IUBA, Oviedo, Spain. [Navazas, Alejandro; Weyens, Nele] Hasselt Univ, Ctr Environm Sci CMK, Hasselt, Belgium. [Lauga, Beatrice] Univ Pau & Pays Adour, Inst Sci Analyt & Physi Chim Environm & Mat IPREM, EEM, UMR5254,CNRS, Pau, France. [Gallego, Jose Luis R.] Univ Oviedo, Dept Explotac & Prospecc Minera IUBA, Mieres, Spain. | - |
local.publisher.place | WASHINGTON | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | UNSP e03411-16 | - |
dc.identifier.doi | 10.1128/AEM.03411-16 | - |
dc.identifier.isi | 000398771200019 | - |
item.contributor | Mesa, Victoria | - |
item.contributor | NAVAZAS, Alejandro | - |
item.contributor | Gonzalez-Gil, Ricardo | - |
item.contributor | Gonzalez, Aida | - |
item.contributor | WEYENS, Nele | - |
item.contributor | Lauga, Beatrice | - |
item.contributor | Gallego, Jose Luis R. | - |
item.contributor | Sanchez, Jesus | - |
item.contributor | Isabel Pelaez, Ana | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2018 | - |
item.fullcitation | Mesa, Victoria; NAVAZAS, Alejandro; Gonzalez-Gil, Ricardo; Gonzalez, Aida; WEYENS, Nele; Lauga, Beatrice; Gallego, Jose Luis R.; Sanchez, Jesus & Isabel Pelaez, Ana (2017) Use of Endophytic and Rhizosphere Bacteria To Improve Phytoremediation of Arsenic-Contaminated Industrial Soils by Autochthonous Betula celtiberica. In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(8), p. e03411-e03416. | - |
item.accessRights | Open Access | - |
crisitem.journal.issn | 0099-2240 | - |
crisitem.journal.eissn | 1098-5336 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FINAL_Main_text_Paper_Betula_AEM_Re AEM02888-16_revised.pdf | Peer-reviewed author version | 978.19 kB | Adobe PDF | View/Open |
Mesa et al., 2017.pdf | Published version | 1.77 MB | Adobe PDF | View/Open |
Supplementary Material Mesa et al., 2017.pdf | Supplementary material | 1.14 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
30
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
77
checked on May 1, 2024
Page view(s)
48
checked on Sep 6, 2022
Download(s)
98
checked on Sep 6, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.