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Title: Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?
Authors: Torres-Farrada, Giselle
Manzano Leon, Ana M.
RINEAU, Francois 
Ledo Alonso, Lucia L.
Sanchez-Lopez, Maria I.
THIJS, Sofie 
Ramos-Leal, Miguel
Guerra, Gilda
Issue Date: 2017
Source: FRONTIERS IN MICROBIOLOGY, 8, p. 1-16 (Art N° 898)
Abstract: White-rot fungi (WRF) and their ligninolytic enzymes (laccases and peroxidases) are considered promising biotechnological tools to remove lignin related Persistent Organic Pollutants from industrial wastewaters and contaminated ecosystems. A high diversity of the genus Ganoderma has been reported in Cuba; in spite of this, the diversity of ligninolytic enzymes and their genes remained unexplored. In this study, 13 native WRF strains were isolated from decayed wood in urban ecosystems in Havana ( Cuba). All strains were identified as Ganoderma sp. using a multiplex polymerase chain reaction (PCR)-method based on ITS sequences. All Ganoderma sp. strains produced laccase enzymes at higher levels than non-specific peroxidases. Native-PAGE of extracellular enzymatic extracts revealed a high diversity of laccase isozymes patterns between the strains, suggesting the presence of different amino acid sequences in the laccase enzymes produced by these Ganoderma strains. We determined the diversity of genes encoding laccases and peroxidases using a PCR and cloning approach with basidiomycete-specific primers. Between two and five laccase genes were detected in each strain. In contrast, only one gene encoding manganese peroxidase or versatile peroxidase was detected in each strain. The translated laccases and peroxidases amino acid sequences have not been described before. Extracellular crude enzymatic extracts produced by the Ganoderma UH strains, were able to degrade model chromophoric compounds such as anthraquinone and azo dyes. These findings hold promises for the development of a practical application for the treatment of textile industry wastewaters and also for bioremediation of polluted ecosystems by well-adapted native WRF strains.
Notes: [Torres-Farrada, Giselle; Ledo Alonso, Lucia L.; Sanchez-Lopez, Maria I.; Guerra, Gilda] Univ Havana, Fac Biol, Dept Microbiol & Virol, Lab Biotechnol, Havana, Cuba. [Manzano Leon, Ana M.; Ramos-Leal, Miguel] Res Inst Trop Fruit Trees, Dept Phytopathol, Havana, Cuba. [Rineau, Francois; Thijs, Sofie; Colpaert, Jan; Vangronsveld, Jaco] Hasselt Univ, Ctr Environm Sci, Environm Biol, Hasselt, Belgium.
Keywords: white-rot fungi; Ganoderma; laccase; manganese peroxidase; versatile peroxidases; bioremediation; chromophoric compounds; Persistent Organic Compounds;white-rot fungi; Ganoderma; laccase; manganese peroxidase; versatile peroxidases; bioremediation; chromophoric compounds; Persistent Organic Compounds
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ISSN: 1664-302X
e-ISSN: 1664-302X
DOI: 10.3389/fmicb.2017.00898
ISI #: 000402235700001
Rights: Copyright © 2017 Torres-Farradá, Manzano León, Rineau, Ledo Alonso, SánchezLópez, Thijs, Colpaert, Ramos-Leal, Guerra and Vangronsveld. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
Category: A1
Type: Journal Contribution
Validations: ecoom 2018
Appears in Collections:Research publications

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