Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24300
Full metadata record
DC FieldValueLanguage
dc.contributor.authorHOSSEINKHANI, Baharak-
dc.contributor.authorvan den Akker, Nynke-
dc.contributor.authorD'HAEN, Jan-
dc.contributor.authorGagliardi, Mick-
dc.contributor.authorSTRUYS, Tom-
dc.contributor.authorLAMBRICHTS, Ivo-
dc.contributor.authorWaltenberger, Johannes-
dc.contributor.authorNelissen, Inge-
dc.contributor.authorHOOYBERGHS, Jef-
dc.contributor.authorMolin, Daniel G. M.-
dc.contributor.authorMICHIELS, Luc-
dc.date.accessioned2017-08-30T09:32:32Z-
dc.date.available2017-08-30T09:32:32Z-
dc.date.issued2017-
dc.identifier.citationNANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 13(5), p. 1663-1671-
dc.identifier.issn1549-9634-
dc.identifier.urihttp://hdl.handle.net/1942/24300-
dc.description.abstractA major conceptual breakthrough in cell signaling has been the finding of EV as new biomarker shuttles in body fluids. Now, one of the major challenges in using these nanometer-sized biological entities as diagnostic marker is the development of translational methodologies to profile them. SPR offers a promising label-free and real time platform with a high potential for biomarker detection. Therefore, we aimed to develop a uniform SPR methodology to detect specific surface markers on EV derived from patient with CHD. EVs having an approximate size range between 30 and 100 nm (similar to 48.5%) and 100-300 nm (similar to 51.5%) were successfully isolated. The biomarker profile of EV was verified using immunogold labeling, ELISA and SPR. Using SPR, we demonstrated an increased binding of EV derived from patients with CHD to anti-ICAM-1 antibodies as compared to EV from healthy donors. Our current findings open up novel opportunities for in-depth and label-free investigation of EV. (C) 2017 The Authors. Published by Elsevier Inc.-
dc.description.sponsorshipThis work was co-financed by the EU through the Interreg IV Flanders-the Netherlands project VaRiA (IVA-VLANED-3.65) and Interreg V Flanders-the Netherlands project Trans Tech Diagnostics (TTD) and Johannes Waltenberger is supported by Cells-in-Motion Cluster of Excellence (EXC 1003-CiM), University of Munster, 48149 Munster, Germany.-
dc.language.isoen-
dc.publisherELSEVIER SCIENCE BV-
dc.rights© 2017 The Authors. Published by Elsevier Inc. Thisis an open access article under theCC BY-NC-ND license-
dc.subject.otherbiosensor; extracellular vesicles; inflammation; cardiovascular disease; endothelial cells; biomarkers-
dc.subject.otherBiosensor; Extracellular vesicles; Inflammation; Cardiovascular disease; Endothelial cells; Biomarkers-
dc.titleDirect detection of nano-scale extracellular vesicles derived from inflammation-triggered endothelial cells using surface plasmon resonance-
dc.typeJournal Contribution-
dc.identifier.epage1671-
dc.identifier.issue5-
dc.identifier.spage1663-
dc.identifier.volume13-
local.format.pages9-
local.bibliographicCitation.jcatA1-
dc.description.notes[Hosseinkhani, Baharak; Struys, Tom; Lambrichts, Ivo; Michiels, Luc] Hasselt Univ, Biomed Res Inst, Martelarenlaan 42, B-3500 Hasselt, Belgium. [D'Haen, Jan] Hasselt Univ, Inst Mat Res, Hasselt, Belgium. [Hooyberghs, Jef] Hasselt Univ, Theoret Phys, Hasselt, Belgium. [van den Akker, Nynke; Gagliardi, Mick; Molin, Daniel G. M.] Maastricht Univ, Dept Physiol, Cardiovasc Res Inst Maastricht CARIM, Maastricht, Netherlands. [Waltenberger, Johannes] Univ Hosp Munster, Dept Cardiovasc Med, Munster, Germany. [Waltenberger, Johannes] Univ Munster, Cells In Mot Cluster Excellence EXC CiM 1003, Munster, Germany. [Hooyberghs, Jef] Flemish Inst Technol Res VITO, Mol, Belgium.-
local.publisher.placeAMSTERDAM-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.classdsPublValOverrule/author_version_not_expected-
dc.identifier.doi10.1016/j.nano.2017.03.010-
dc.identifier.isi000403662500009-
item.fullcitationHOSSEINKHANI, Baharak; van den Akker, Nynke; D'HAEN, Jan; Gagliardi, Mick; STRUYS, Tom; LAMBRICHTS, Ivo; Waltenberger, Johannes; Nelissen, Inge; HOOYBERGHS, Jef; Molin, Daniel G. M. & MICHIELS, Luc (2017) Direct detection of nano-scale extracellular vesicles derived from inflammation-triggered endothelial cells using surface plasmon resonance. In: NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 13(5), p. 1663-1671.-
item.fulltextWith Fulltext-
item.accessRightsOpen Access-
item.validationecoom 2018-
item.contributorHOSSEINKHANI, Baharak-
item.contributorvan den Akker, Nynke-
item.contributorNelissen, Inge-
item.contributorWaltenberger, Johannes-
item.contributorHOOYBERGHS, Jef-
item.contributorLAMBRICHTS, Ivo-
item.contributorSTRUYS, Tom-
item.contributorMolin, Daniel G. M.-
item.contributorGagliardi, Mick-
item.contributorD'HAEN, Jan-
item.contributorMICHIELS, Luc-
crisitem.journal.issn1549-9634-
crisitem.journal.eissn1549-9642-
Appears in Collections:Research publications
Files in This Item:
File Description SizeFormat 
aa.pdfPublished version1.09 MBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

13
checked on Sep 5, 2020

WEB OF SCIENCETM
Citations

23
checked on Jul 7, 2022

Page view(s)

92
checked on Jun 1, 2022

Download(s)

172
checked on Jun 1, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.