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http://hdl.handle.net/1942/24351
Title: | A novel, highly conserved metallothionein family in basidiomycete fungi and characterization of two representative SlMTa and SlMTb genes in the ectomycorrhizal fungus Suillus luteus | Authors: | NGUYEN, Hoai RINEAU, Francois VANGRONSVELD, Jaco CUYPERS, Ann COLPAERT, Jan RUYTINX, Joske |
Issue Date: | 2017 | Publisher: | WILEY | Source: | ENVIRONMENTAL MICROBIOLOGY, 19(7), p. 2577-2587 | Abstract: | The basidiomycete Suillus luteus is an important member of the ectomycorrhizal community that thrives in heavy metal polluted soils covered with pioneer pine forests. This study aimed to identify potential heavy metal chelators in S. luteus. Two metallothionein (MT) coding genes, SlMTa and SlMTb, were identified. When heterologously expressed in yeast, both SlMTa and SlMTb can rescue the Cu sensitive mutant from Cu toxicity. In S. luteus, transcription of both SlMTa and SlMTb is induced by Cu but not Cd or Zn. Several putative Cu-sensing and metal-response elements are present in the promoter sequences. These results indicate that SlMTa and SlMTb function as Cu-thioneins. Homologs of the S. luteus MTs are present in 49 species belonging to 10 different orders of the subphylum Agaricomycotina and are remarkably conserved. The length of the proteins, number and distribution of cysteine residues indicate a novel family of fungal MTs. The ubiquitous and highly conserved features of these MTs suggest that they are important for basic cellular functions in species in the subphylum Agaricomycotina. | Notes: | [Hoai Nguyen; Rineau, Francois; Vangronsveld, Jaco; Cuypers, Ann; Colpaert, Jan V.; Ruytinx, Joske] Hasselt Univ, Ctr Environm Sci, Environm Biol, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. | Document URI: | http://hdl.handle.net/1942/24351 | ISSN: | 1462-2912 | e-ISSN: | 1462-2920 | DOI: | 10.1111/1462-2920.13729 | ISI #: | 000405907300007 | Rights: | (c) 2017 Society for Applied Microbiology and John Wiley & Sons Ltd | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2018 |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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nguyen 1.pdf Restricted Access | Published version | 942.89 kB | Adobe PDF | View/Open Request a copy |
Nguyen et al. 2017_auteursversie.pdf | Peer-reviewed author version | 1.48 MB | Adobe PDF | View/Open |
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