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Title: | Identification, evolution and functional characterization of two Zn CDF-family transporters of the ectomycorrhizal fungus Suillus luteus | Authors: | RUYTINX, Joske CONINX, Laura NGUYEN, Hoai SMISDOM, Nick Morin, Emmanuelle Kohler, Annegret CUYPERS, Ann COLPAERT, Jan |
Issue Date: | 2017 | Publisher: | WILEY | Source: | ENVIRONMENTAL MICROBIOLOGY REPORTS, 9(4), p. 419-427 | Abstract: | Two genes, SlZnT1 and SlZnT2, encoding Cation Diffusion Facilitator (CDF) family transporters were isolated from Suillus luteus mycelium by genome walking. Both gene models are very similar and phylogenetic analysis indicates that they are most likely the result of a recent gene duplication event. Comparative sequence analysis of the deduced proteins predicts them to be Zn transporters. This function was confirmed by functional analysis in yeast for SlZnT1. SlZnT1 was able to restore growth of the highly Zn sensitive yeast mutant zrc1 and localized to the vacuolar membrane. Transformation of zrc1 yeast cells with SlZnT1 resulted in an increased accumulation of Zn compared to empty vector transformed zrc1 yeast cells and equals Zn accumulation in wild type yeast cells. We were not able to express functional SlZnT2 in yeast. In S. luteus, both SlZnT genes are constitutively expressed whatever the external Zn concentrations. A labile Zn pool was detected in the vacuoles of S. luteus free-living mycelium. Therefore we conclude that SlZnT1 is indispensable for maintenance of Zn homeostasis by transporting excess Zn into the vacuole. | Notes: | [Ruytinx, Joske; Coninx, Laura; Nguyen, Hoai; Cuypers, Ann; Colpaert, Jan V.] Hasselt Univ, Ctr Environm Sci, Environm Biol, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Smisdom, Nick] Hasselt Univ, Biomed Res Inst, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Morin, Emmanuelle; Kohler, Annegret] Univ Lorraine Interact Arbres Microorganismes, Inst Natl Rech Agron, Lab Excellence ARBRE, UMR1136, F-54280 Champenoux, France. | Document URI: | http://hdl.handle.net/1942/24371 | ISSN: | 1758-2229 | e-ISSN: | 1758-2229 | DOI: | 10.1111/1758-2229.12551 | ISI #: | 000405931900010 | Rights: | (C) 2017 Society for Applied Microbiology and John Wiley & Sons Ltd | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2018 |
Appears in Collections: | Research publications |
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Ruytinx_et_al-2017-Environmental_Microbiology_Reports (1).pdf Restricted Access | Published version | 717.92 kB | Adobe PDF | View/Open Request a copy |
Ruytinx et al_2017_CDF.pdf | Peer-reviewed author version | 3.97 MB | Adobe PDF | View/Open |
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