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Title: | Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure | Authors: | WINCKELMANS, Ellen NAWROT, Tim TSAMOU, Maria Den Hond, Elly Baeyens, Willy KLEINJANS, J. Lefebvre, Wouter Van Larebeke, Nicolas PEUSENS, Martien PLUSQUIN, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte DE KOK, Theo VRIJENS, Karen |
Issue Date: | 2017 | Publisher: | BIOMED CENTRAL LTD | Source: | ENVIRONMENTAL HEALTH, 16, p. 1-15 (Art N° 87) | Abstract: | Background: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short-and medium-term PM10 exposure. Methods: Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results: Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions: In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. | Notes: | [Winckelmans, Ellen; Nawrot, Tim S.; Tsamou, Maria; Peusens, Martien; Plusquin, Michelle; Vrijens, Karen] Hasselt Univ, Ctr Environm Sci, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium. [Nawrot, Tim S.] Leuven Univ, Dept Publ Hlth & Primary Care, Leuven, Belgium. [Den Hond, Elly] Prov Inst Hyg, Antwerp, Belgium. [Baeyens, Willy] Vrije Univ Brussel, Dept Analyt & Environm Chem, Brussels, Belgium. [Kleinjans, Jos; de Kok, Theo M.] Maastricht Univ, Dept Toxicogen, Maastricht, Netherlands. [Lefebvre, Wouter; Schoeters, Greet] Flemish Inst Technol Res, Mol, Belgium. [Van Larebeke, Nicolas] Univ Ghent, Dept Radiotherapy & Nucl Med, Ghent, Belgium. [Reynders, Hans] Flemish Govt, Nat & Energy Dept, Environm, Brussels, Belgium. [Schoeters, Greet] Univ Antwerp, Dept Biomed Sci, Antwerp, Belgium. [Schoeters, Greet] Univ Southern Denmark, Dept Environm Med, Inst Publ Hlth, Odense, Denmark. [Vanpoucke, Charlotte] Belgian Interreg Environm Agcy IRCEL, Brussels, Belgium. | Keywords: | Ambient air pollution; Particulate matter; Transcriptome-wide analyses; Sex-specific; mitochondria;ambient air pollution; particulate matter; transcriptome-wide analyses; sex-specific; mitochondria | Document URI: | http://hdl.handle.net/1942/24462 | e-ISSN: | 1476-069X | DOI: | 10.1186/s12940-017-0292-7 | ISI #: | 000408044200001 | Rights: | © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2018 |
Appears in Collections: | Research publications |
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