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http://hdl.handle.net/1942/2464
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | WENMACKERS, Sylvia | - |
dc.contributor.author | HAENEN, Ken | - |
dc.contributor.author | NESLADEK, Milos | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.contributor.author | MICHIELS, Luc | - |
dc.contributor.author | VAN DE VEN, Martin | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.date.accessioned | 2007-11-14T10:55:06Z | - |
dc.date.available | 2007-11-14T10:55:06Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | PHYSICA STATUS SOLIDI A-APPLIED RESEARCH, 199(1). p. 44-48 | - |
dc.identifier.issn | 0031-8965 | - |
dc.identifier.uri | http://hdl.handle.net/1942/2464 | - |
dc.description.abstract | Chemical vapour deposited (CVD) diamond is used in DNA immobilization experiments for its high stability and versatility. In this study a double-stranded, 250 base pairs long DNA fragment of the human PKU gene was covalently bound to CVD diamond films. Thymidine was used as a linker molecule for the binding in head-on configuration. The presence of surface-bound FITC-labelled DNA was confirmed by confocal fluorescence microscopy. In denaturation experiments the second, unlabelled strand of each pair was removed resulting in a loss of fluorescence. The possibility of non-specific binding of DNA to diamond can be excluded. (C) 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Covalent immobilization of DNA on CVD diamond films | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 48 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 44 | - |
dc.identifier.volume | 199 | - |
local.format.pages | 5 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Limburgs Univ Centrum, Mat Res Inst, B-3590 Diepenbeek, Belgium. Linburgs Univ Centrum, Biomed Res Inst, B-3590 Diepenbeek, Belgium.Wenmackers, S, Limburgs Univ Centrum, Mat Res Inst, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1002/pssa.200303822 | - |
dc.identifier.isi | 000185422000010 | - |
item.validation | ecoom 2004 | - |
item.contributor | WENMACKERS, Sylvia | - |
item.contributor | HAENEN, Ken | - |
item.contributor | NESLADEK, Milos | - |
item.contributor | WAGNER, Patrick | - |
item.contributor | MICHIELS, Luc | - |
item.contributor | VAN DE VEN, Martin | - |
item.contributor | AMELOOT, Marcel | - |
item.accessRights | Closed Access | - |
item.fullcitation | WENMACKERS, Sylvia; HAENEN, Ken; NESLADEK, Milos; WAGNER, Patrick; MICHIELS, Luc; VAN DE VEN, Martin & AMELOOT, Marcel (2003) Covalent immobilization of DNA on CVD diamond films. In: PHYSICA STATUS SOLIDI A-APPLIED RESEARCH, 199(1). p. 44-48. | - |
item.fulltext | No Fulltext | - |
crisitem.journal.issn | 0031-8965 | - |
crisitem.journal.eissn | 1862-6319 | - |
Appears in Collections: | Research publications |
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