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http://hdl.handle.net/1942/2465
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DC Field | Value | Language |
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dc.contributor.author | WIEHART, Ursula | - |
dc.contributor.author | Nicolson, SW | - |
dc.contributor.author | VAN KERKHOVE, Emmy | - |
dc.date.accessioned | 2007-11-14T10:55:59Z | - |
dc.date.available | 2007-11-14T10:55:59Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | JOURNAL OF INSECT PHYSIOLOGY, 49(10). p. 955-965 | - |
dc.identifier.issn | 0022-1910 | - |
dc.identifier.uri | http://hdl.handle.net/1942/2465 | - |
dc.description.abstract | The Malpighian tubules of Tenebrio molitor provide a model system for interpreting the actions of endogenous diuretic and antidiuretic peptides. The effects of diuretic (Tenmo-DH37) and antidiuretic (Tenmo-ADFa) peptides and their respective second messengers (cyclic AMP and cyclic GMP) on basolateral (V-bl) and transepithelial (V-te) potentials of Tenebrio Malpighian tubules were determined using conventional microelectrodes. In the presence of 6 mmol l(-1) Ba2+, Tenmo-DH37 (100 nmol l(-1)) reversibly hyperpolarized V, and depolarized Y-te. A similar response was seen with the addition of I mmol l(-1) cyclic AMP; however, the apical membrane potential (V-ap) then showed a hyperpolarization, whereas a depolarization of Vap was observed with Tenmo-DH37Bafilomycin A(1) (5 mumol l(-1)) inhibited fluid secretion of stimulated tubules and reversed the hyperpolarization of V-bl in response to Tenmo-DH37, In response to 100 nmol l(-1) Tenmo-ADFa or 1 mmol l(-1) cyclic GMP, V-bl and V-te depolarized, although cyclic GMP affected membrane potentials somewhat differently by causing an initial hyperpolarization of V-bl and V-te. In high [K+-]-low [Na+] Ringer, 1 mmol l(-1) amiloride decreased fluid secretion rates, and depolarized both V-bl and V-te. Amiloride significantly decreased luminal pH in paired experiments, indicating the presence of a K+/nH(+) exchanger in tubule cells of Tenebrio. The results suggest that the endogenous factors and their second messengers stimulate/inhibit fluid secretion by acting on the apical V-ATPase, basolateral K+ transport, and possibly Cl- transport. (C) 2003 Elsevier Ltd. All rights reserved. | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject.other | endogenous factors; diuretic peptides; antidiaretic peptides; second messengers; Malpighian tubules; Tenebrio molitor; electrophysiology | - |
dc.title | The effects of endogenous diuretic and antidiuretic peptides and their second messengers in the Malpighian tubules of Tenebrio molitor: an electrophysiological study | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 965 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 955 | - |
dc.identifier.volume | 49 | - |
local.format.pages | 11 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Limburgs Univ Ctr, Physiol Lab, B-3590 Diepenbeek, Belgium. Univ Pretoria, Dept Zool & Entomol, ZA-0002 Pretoria, South Africa.Van Kerkhove, E, Limburgs Univ Ctr, Physiol Lab, Univ Campus Gebouw D, B-3590 Diepenbeek, Belgium. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/S0022-1910(03)00165-3 | - |
dc.identifier.isi | 000185965800008 | - |
item.fulltext | No Fulltext | - |
item.fullcitation | WIEHART, Ursula; Nicolson, SW & VAN KERKHOVE, Emmy (2003) The effects of endogenous diuretic and antidiuretic peptides and their second messengers in the Malpighian tubules of Tenebrio molitor: an electrophysiological study. In: JOURNAL OF INSECT PHYSIOLOGY, 49(10). p. 955-965. | - |
item.contributor | WIEHART, Ursula | - |
item.contributor | Nicolson, SW | - |
item.contributor | VAN KERKHOVE, Emmy | - |
item.accessRights | Closed Access | - |
item.validation | ecoom 2004 | - |
crisitem.journal.issn | 0022-1910 | - |
crisitem.journal.eissn | 1879-1611 | - |
Appears in Collections: | Research publications |
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