Antioxidative responses of wheat treated with realistic concentration of cadmium

Abstract

Two cvs. of wheat differently sensitive to many stress factors (cv. Ofanto less sensitive than cv. Adamello) were grown in a controlled environment with cadmium near threshold concentrations supplying the metal at equal-effect concentrations. Cd excess determined in both cvs. a reduction in water and turgor potential but a maintenance of relative water content. Cv Ofanto showed a higher capacity of Cd exclusion from roots but a higher translocation to shoots in comparison with cv. Adamello. Notwithstanding the higher metal concentration in leaves of cv. Ofanto, K+ leakage was more pronounced in Adamello suggesting that mechanisms of Cd detoxification and tolerance such as vacuolar compartmentalisation were activated in the first one. In Adamello plants, ethylene rose at the lowest metal concentration and the activation in roots of the antioxidative enzymes catalase, ascorbate peroxidase and guaiacol peroxidase came into play whereas in Ofanto ethylene and catalase did not change. Following cadmium treatment, superoxide dismutase activity was reduced or remained at the control value in roots and in leaves. For both cultivars ascorbate peroxidase, syringaldazine peroxidase and guaiacol peroxidase activities were always higher in roots than in leaves. These activities were induced by Cd in Ofanto leaves, whereas in Adamello leaves they remained at control levels or increased somewhat at the highest metal concentration. Cadmium changed the peroxidase isozyme pattern in both cultivars. Cv. Ofanto showed, as for other stress such as drought, salinity, nickel and copper, a co-tolerance towards Cd. Analogies in the response to other metals such as copper could be found in activation of catalase at the lower metal concentration in cv. Adamello and in the induction of ascorbate peroxidase in leaves of cv. Ofanto. (C) 2003 Elsevier B.V. All rights reserved.