Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/24987
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cattoir, Lien | - |
dc.contributor.author | Van Hoecke, Frederik | - |
dc.contributor.author | Van Maerken, Tom | - |
dc.contributor.author | Nys, Eveline | - |
dc.contributor.author | Ryckaert, Inge | - |
dc.contributor.author | De Boulle, Matthias | - |
dc.contributor.author | Geerts, Anja | - |
dc.contributor.author | Verhelst, Xavier | - |
dc.contributor.author | Colle, Isabelle | - |
dc.contributor.author | Hutse, Veronik | - |
dc.contributor.author | Suin, Vanessa | - |
dc.contributor.author | Wautier, Magali | - |
dc.contributor.author | Van Gucht, Steven | - |
dc.contributor.author | Van Vlierberghe, Hans | - |
dc.contributor.author | PADALKO, Elizaveta | - |
dc.date.accessioned | 2017-10-10T13:33:30Z | - |
dc.date.available | 2017-10-10T13:33:30Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | ARCHIVES OF VIROLOGY, 162(9), p. 2625-2632 | - |
dc.identifier.issn | 0304-8608 | - |
dc.identifier.uri | http://hdl.handle.net/1942/24987 | - |
dc.description.abstract | Hepatitis E virus (HEV) genotype 3 is an emerging pathogen in the developed world. As the clinical manifestations and routine laboratory parameters are often nonspecific, accurate diagnostic tests are crucial. In the current study, the performance of six serological assays and three PCR assays for the detection of HEV was evaluated. In the setting of the Ghent University Hospital, patients with clinically suspected HEV infection were tested for the presence of HEV IgM and IgG as well as HEV RNA. Serology was performed using six commercial HEV ELISA assays: Biorex, Wantai and Mikrogen IgM and IgG. HEV RNA was detected using one commercial assay (Altona RealStarA (R)), and two optimized in-house real-time RT-PCR assays (according to Jothikumar et al., 2006 and Gyarmati et al., 2007). In addition, all three PCR assays were performed on 16 external quality control (EQC) samples. In a period of 39 months (January 2011-April 2014), 70 patients were enrolled. Using different ELISA assays, the prevalence of antibodies varied from 5.7% to 14.3% for HEV IgM and from 15.7% to 20.0% for IgG. All but two of the results of the PCR assays performed on clinical samples agreed. However, 10 out of 16 EQC samples results showed major discrepancies. We observed important differences in the performance of various serological and PCR assays. For this reason, results of both serological and molecular tests for HEV should be interpreted with caution. | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER WIEN | - |
dc.rights | (c) Springer-Verlag Wien 2017 | - |
dc.title | Hepatitis E virus serology and PCR: does the methodology matter? | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 2632 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 2625 | - |
dc.identifier.volume | 162 | - |
local.format.pages | 8 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Cattoir, Lien; Van Hoecke, Frederik; Van Maerken, Tom; Nys, Eveline; Ryckaert, Inge; Padalko, Elizaveta] Ghent Univ Hosp, Dept Clin Chem Microbiol & Immunol, De Pintelaan 185,2P8, B-9000 Ghent, Belgium. [De Boulle, Matthias; Geerts, Anja; Verhelst, Xavier; Colle, Isabelle; Van Vlierberghe, Hans] Ghent Univ Hosp, Dept Hepatol & Gastroenterol, Ghent, Belgium. [Hutse, Veronik; Suin, Vanessa; Wautier, Magali; Van Gucht, Steven] Sci Inst Publ Hlth, Natl Reference Ctr Viral Hepatitis, Brussels, Belgium. [Padalko, Elizaveta] Hasselt Univ, Sch Life Sci, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. | - |
local.publisher.place | WIEN | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1007/s00705-017-3395-0 | - |
dc.identifier.isi | 000408059500008 | - |
item.contributor | Cattoir, Lien | - |
item.contributor | Van Hoecke, Frederik | - |
item.contributor | Van Maerken, Tom | - |
item.contributor | Nys, Eveline | - |
item.contributor | Ryckaert, Inge | - |
item.contributor | De Boulle, Matthias | - |
item.contributor | Geerts, Anja | - |
item.contributor | Verhelst, Xavier | - |
item.contributor | Colle, Isabelle | - |
item.contributor | Hutse, Veronik | - |
item.contributor | Suin, Vanessa | - |
item.contributor | Wautier, Magali | - |
item.contributor | Van Gucht, Steven | - |
item.contributor | Van Vlierberghe, Hans | - |
item.contributor | PADALKO, Elizaveta | - |
item.validation | ecoom 2018 | - |
item.fullcitation | Cattoir, Lien; Van Hoecke, Frederik; Van Maerken, Tom; Nys, Eveline; Ryckaert, Inge; De Boulle, Matthias; Geerts, Anja; Verhelst, Xavier; Colle, Isabelle; Hutse, Veronik; Suin, Vanessa; Wautier, Magali; Van Gucht, Steven; Van Vlierberghe, Hans & PADALKO, Elizaveta (2017) Hepatitis E virus serology and PCR: does the methodology matter?. In: ARCHIVES OF VIROLOGY, 162(9), p. 2625-2632. | - |
item.accessRights | Restricted Access | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 0304-8608 | - |
crisitem.journal.eissn | 1432-8798 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hep.pdf Restricted Access | Published version | 595.93 kB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
9
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
11
checked on Apr 22, 2024
Page view(s)
48
checked on Sep 6, 2022
Download(s)
44
checked on Sep 6, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.