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Title: Biosensing based on light absorption of nanoscaled gold and silver particles
Authors: FREDERIX, Filip 
Friedt, JM
Choi, KH
Laureyn, W
Campitelli, A
Maes, Guido
Borghs, G
Issue Date: 2003
Source: ANALYTICAL CHEMISTRY, 75(24). p. 6894-6900
Abstract: The absorption spectrum of noble metal spherical nanoparticles is known to be strongly influenced by the dielectric constant of the surrounding material, and as such, these particles are well suited for biosensing applications. To perform biosensing using nanoparticles on a substrate, the metal particles are covalently attached onto quartz using an organic adhesion layer of mercaptosilanes. The particles in solution are characterized by UV-vis spectroscopy and transmission electron microscopy, while those attached to the quartz are characterized with UV-vis spectroscopy and atomic force microscopy. Antibodies are attached to the metal nanoparticles, and the antigen recognition is monitored via the change of light absorption when this binding event occurs. Not only is the absorbance originating from plasmon resonances of the particles influenced by the dielectric properties of molecules attached to the nanospheres but also the interband absorption of the particles changes, which will be demonstrated in this report. A light absorption change is detected when a molecular recognition occurs between the bioreceptor molecules attached to the nanoparticle and a biomolecular counterpart. This change in absorption can be very large when adhered molecules are at resonance (interband transitions). In addition, the presented type of biosensing can be a cost-effective and easy to use alternative to conventional biosensing techniques.
Notes: IMEC VZW, MCP BIO, B-3001 Louvain, Belgium. Limburgs Univ Ctr, Lab Inorgan & Phys Chem, IMO, B-3590 Diepenbeek, Belgium. Katholieke Univ Leuven, Dept Chem, B-3001 Louvain, Belgium.Frederix, F, IMEC VZW, MCP BIO, Kapeldreef 75, B-3001 Louvain, Belgium.
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ISSN: 0003-2700
e-ISSN: 1520-6882
DOI: 10.1021%2Fac0346609
ISI #: 000187283000018
Category: A1
Type: Journal Contribution
Validations: ecoom 2005
Appears in Collections:Research publications

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