Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/25490
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dc.contributor.authorWOLFS, Esther-
dc.contributor.authorHolvoet, Bryan-
dc.contributor.authorOrdovas, Laura-
dc.contributor.authorBreuls, Natacha-
dc.contributor.authorHelsen, Nicky-
dc.contributor.authorSchonberger, Matthias-
dc.contributor.authorRaitano, Susanna-
dc.contributor.authorSTRUYS, Tom-
dc.contributor.authorVanbilloen, Bert-
dc.contributor.authorCasteels, Cindy-
dc.contributor.authorSampaolesi, Maurilio-
dc.contributor.authorVan Laere, Koen-
dc.contributor.authorLAMBRICHTS, Ivo-
dc.contributor.authorVerfaillie, Catherine M.-
dc.contributor.authorDeroose, Christophe M.-
dc.date.accessioned2018-01-30T14:18:12Z-
dc.date.available2018-01-30T14:18:12Z-
dc.date.issued2017-
dc.identifier.citationJOURNAL OF NUCLEAR MEDICINE, 58(10), p. 1659-1665-
dc.identifier.issn0161-5505-
dc.identifier.urihttp://hdl.handle.net/1942/25490-
dc.description.abstractMolecular imaging is indispensable for determining the fate and persistence of engrafted stem cells. Standard strategies for transgene induction involve the use of viral vectors prone to silencing and insertional mutagenesis or the use of nonhuman genes. Methods: We used zinc finger nucleases to induce stable expression of human imaging reporter genes into the safe-harbor locus adeno-associated virus integration site 1 in human embryonic stem cells. Plasmids were generated carrying reporter genes for fluorescence, bioluminescence imaging, and human PET reporter genes. Results: In vitro assays confirmed their functionality, and embryonic stem cells retained differentiation capacity. Teratoma formation assays were performed, and tumors were imaged over time with PET and bioluminescence imaging. Conclusion: This study demonstrates the application of genome editing for targeted integration of human imaging reporter genes in human embryonic stem cells for longterm molecular imaging.-
dc.description.sponsorshipChristophe M. Deroose and Koen Van Laere are senior clinical investigators of the Flemish Fund for Scientific Research (FWO). Cindy Casteels is a postdoctoral fellow of FWO. Bryan Holvoet and Natacha Breuls are PhD students funded by IWT. Laura Ordovas was funded by IWT (IWT/OZM/090838), IACS (BPAMER3/08/04), and the Government of Aragon (FMI048/08). Funding to Christophe M. Deroose was from FWO (G.0667.07 and G.0975.11), KU Leuven (ETH-C1900-PF and EME-C2161-GOA/11/012), IWT (IWT-HEPSTEM and IWT-HILIM-3D), BELSPO (BELSPO-IUAP-DEVREPAIR), and HeMiBio (FP7-HEALTH, project 266777). No other potential conflict of interest relevant to this article was reported-
dc.language.isoen-
dc.publisherSOC NUCLEAR MEDICINE INC-
dc.subject.othergenome editing; stem cells; reporter genes; PET; noninvasive imaging-
dc.subject.othergenome editing; stem cells; reporter genes; PET; noninvasive imaging-
dc.titleMolecular Imaging of Human Embryonic Stem Cells Stably Expressing Human PET Reporter Genes After Zinc Finger Nuclease-Mediated Genome Editing-
dc.typeJournal Contribution-
dc.identifier.epage1665-
dc.identifier.issue10-
dc.identifier.spage1659-
dc.identifier.volume58-
local.format.pages7-
local.bibliographicCitation.jcatA1-
dc.description.notes[Wolfs, Esther; Holvoet, Bryan; Breuls, Natacha; Vanbilloen, Bert; Casteels, Cindy; Van Laere, Koen; Deroose, Christophe M.] Katholieke Univ Leuven, Dept Imaging & Pathol, Mol Small Anim Imaging Ctr, Nucl Med & Mol Imaging, Leuven, Belgium. [Ordovas, Laura; Helsen, Nicky; Raitano, Susanna; Verfaillie, Catherine M.] Katholieke Univ Leuven, Stem Cell Inst, Leuven, Belgium. [Ordovas, Laura; Helsen, Nicky; Raitano, Susanna; Verfaillie, Catherine M.] Katholieke Univ Leuven, Stem Cell Biol & Embryol, Dept Dev & Regenerat, Leuven, Belgium. [Breuls, Natacha; Sampaolesi, Maurilio] Katholieke Univ Leuven, Translat Cardiomyol Lab, Dept Dev & Regenerat, Leuven, Belgium. [Schonberger, Matthias] Katholieke Univ Leuven, Dept Pharmaceut & Pharmacol Sci, Leuven, Belgium. [Struys, Tom; Lambrichts, Ivo] Univ Hasselt, Biomed Res Inst, Morphol Res Grp, Histol Lab, Diepenbeek, Belgium.-
local.publisher.placeRESTON-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.2967/jnumed.117.189779-
dc.identifier.isi000411985900025-
item.accessRightsRestricted Access-
item.validationecoom 2018-
item.fulltextWith Fulltext-
item.fullcitationWOLFS, Esther; Holvoet, Bryan; Ordovas, Laura; Breuls, Natacha; Helsen, Nicky; Schonberger, Matthias; Raitano, Susanna; STRUYS, Tom; Vanbilloen, Bert; Casteels, Cindy; Sampaolesi, Maurilio; Van Laere, Koen; LAMBRICHTS, Ivo; Verfaillie, Catherine M. & Deroose, Christophe M. (2017) Molecular Imaging of Human Embryonic Stem Cells Stably Expressing Human PET Reporter Genes After Zinc Finger Nuclease-Mediated Genome Editing. In: JOURNAL OF NUCLEAR MEDICINE, 58(10), p. 1659-1665.-
item.contributorWOLFS, Esther-
item.contributorHolvoet, Bryan-
item.contributorOrdovas, Laura-
item.contributorBreuls, Natacha-
item.contributorHelsen, Nicky-
item.contributorSchonberger, Matthias-
item.contributorRaitano, Susanna-
item.contributorSTRUYS, Tom-
item.contributorVanbilloen, Bert-
item.contributorCasteels, Cindy-
item.contributorSampaolesi, Maurilio-
item.contributorVan Laere, Koen-
item.contributorLAMBRICHTS, Ivo-
item.contributorVerfaillie, Catherine M.-
item.contributorDeroose, Christophe M.-
crisitem.journal.issn0161-5505-
crisitem.journal.eissn1535-5667-
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