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http://hdl.handle.net/1942/25505
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DC Field | Value | Language |
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dc.contributor.author | DONDERS, Raf | - |
dc.contributor.author | BOGIE, Jeroen | - |
dc.contributor.author | RAVANIDIS, Stelios | - |
dc.contributor.author | GERVOIS, Pascal | - |
dc.contributor.author | VANHEUSDEN, Marjan | - |
dc.contributor.author | Marée, Raphaël | - |
dc.contributor.author | Schrynemackers, Marie | - |
dc.contributor.author | Smeets, Hubert J.M. | - |
dc.contributor.author | Pinxteren, Jef | - |
dc.contributor.author | Gijbels, Kristel | - |
dc.contributor.author | Walbers, Sara | - |
dc.contributor.author | Mays, Robert W. | - |
dc.contributor.author | Deans, Robert | - |
dc.contributor.author | Van Den Bosch, Ludo | - |
dc.contributor.author | STINISSEN, Piet | - |
dc.contributor.author | LAMBRICHTS, Ivo | - |
dc.contributor.author | GYSELAERS, Wilfried | - |
dc.contributor.author | HELLINGS, Niels | - |
dc.date.accessioned | 2018-02-12T13:55:21Z | - |
dc.date.available | 2018-02-12T13:55:21Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Stem cells and development, 27(2), p. 65-84 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | http://hdl.handle.net/1942/25505 | - |
dc.description.abstract | Mesenchymal stromal cells (MSCs) are multipotent stem cells with immunosuppressive and trophic support functions. While MSCs from different sources frequently display a similar appearance in culture, they often show differences in their surface marker and gene expression profiles. Although bone marrow is considered the ‘‘gold standard’’ tissue to isolate classical MSCs (BM-MSC), MSC-like cells are currently also derived from more easily accessible extra-embryonic tissues such as the umbilical cord. In this study, we defined the best way to isolate MSCs from the Wharton’s jelly of the human umbilical cord (WJ-MSC) and assessed the mesenchymal and immunological phenotype of BM-MSC and WJ-MSC. Moreover, the gene expression profile of established WJ-MSC cultures was compared to two different bone marrow-derived stem cell populations (BM-MSC and multipotent adult progenitor cells or MAPC). We observed that explant culturing of Wharton’s jelly matrix is superior to collagenase tissue digestion for obtaining mesenchymal-like cells, with explant isolated cells displaying increased expansion potential. While being phenotypically similar to adult MSCs, WJ-MSC show a different gene expression profile. Gene ontology analysis revealed that genes associated with cell adhesion, proliferation, and immune system functioning are enriched in WJ-MSC. In vivo transplantation confirms their immune modulatory effect on T cells, similar to BMMSC and MAPC. Furthermore, WJ-MSC intrinsically overexpress genes involved in neurotrophic support and their secretome induces neuronal maturation of SH-SY5Y neuroblastoma cells to a greater extent than BM-MSC. This signature makes WJ-MSC an attractive candidate for cell-based therapy in neurodegenerative and immune-mediated central nervous system disorders such as multiple sclerosis, Parkinson’s disease, or amyotrophic lateral sclerosis. | - |
dc.description.sponsorship | This research was promoted by grants from the IWT (agentschap voor Innovatie door Wetenschap en Technology), Alma-in-Silico (EMR INT4.-1.3.-2008-03/003), the VIB (Vlaams Instituut voor Biotechnologie), the transnational University Limburg, Hasselt University, and Limburg Sterk Merk. Pascal Gervois is supported by grant number 12 U7718 N of the Fonds Wetenschappelijk Onderzoek Vlaanderen. | - |
dc.language.iso | en | - |
dc.rights | (C) Mary Ann Liebert, Inc. | - |
dc.subject.other | umbilical cord; microarray; MAPC; MSC; neurotrophic factors; immune modulation | - |
dc.title | Human Wharton's Jelly-Derived Stem Cells Display a Distinct Immunomodulatory and Proregenerative Transcriptional Signature Compared to Bone Marrow-Derived Stem Cells | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 84 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 65 | - |
dc.identifier.volume | 27 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.class | dsPublValOverrule/author_version_not_expected | - |
dc.identifier.doi | 10.1089/scd.2017.0029 | - |
dc.identifier.isi | 000418565900001 | - |
item.validation | ecoom 2019 | - |
item.contributor | DONDERS, Raf | - |
item.contributor | BOGIE, Jeroen | - |
item.contributor | RAVANIDIS, Stelios | - |
item.contributor | GERVOIS, Pascal | - |
item.contributor | VANHEUSDEN, Marjan | - |
item.contributor | Marée, Raphaël | - |
item.contributor | Schrynemackers, Marie | - |
item.contributor | Smeets, Hubert J.M. | - |
item.contributor | Pinxteren, Jef | - |
item.contributor | Gijbels, Kristel | - |
item.contributor | Walbers, Sara | - |
item.contributor | Mays, Robert W. | - |
item.contributor | Deans, Robert | - |
item.contributor | Van Den Bosch, Ludo | - |
item.contributor | STINISSEN, Piet | - |
item.contributor | LAMBRICHTS, Ivo | - |
item.contributor | GYSELAERS, Wilfried | - |
item.contributor | HELLINGS, Niels | - |
item.accessRights | Open Access | - |
item.fullcitation | DONDERS, Raf; BOGIE, Jeroen; RAVANIDIS, Stelios; GERVOIS, Pascal; VANHEUSDEN, Marjan; Marée, Raphaël; Schrynemackers, Marie; Smeets, Hubert J.M.; Pinxteren, Jef; Gijbels, Kristel; Walbers, Sara; Mays, Robert W.; Deans, Robert; Van Den Bosch, Ludo; STINISSEN, Piet; LAMBRICHTS, Ivo; GYSELAERS, Wilfried & HELLINGS, Niels (2018) Human Wharton's Jelly-Derived Stem Cells Display a Distinct Immunomodulatory and Proregenerative Transcriptional Signature Compared to Bone Marrow-Derived Stem Cells. In: Stem cells and development, 27(2), p. 65-84. | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 1547-3287 | - |
crisitem.journal.eissn | 1557-8534 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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scd.2017.0029.pdf Restricted Access | Published version | 888.54 kB | Adobe PDF | View/Open Request a copy |
draft for resubmission check.pdf | Non Peer-reviewed author version | 2.38 MB | Adobe PDF | View/Open |
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