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Title: | Engineered neural tissue with Schwann cell differentiated human dental pulp stem cells: potential for peripheral nerve repair? | Authors: | SANEN, Kathleen MARTENS, Wendy Georgiou, Melanie AMELOOT, Marcel LAMBRICHTS, Ivo Phillips, James |
Issue Date: | 2017 | Publisher: | WILEY | Source: | JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 11(12), p. 3362-3372 | Abstract: | Despite the spontaneous regenerative capacity of the peripheral nervous system, large gap peripheral nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow nerve conduits in the clinic urge the need for alternative treatments. Recently, we have described promising neuroregenerative capacities of Schwann cells derived from differentiated human dental pulp stem cells (d-hDPSCs) in vitro. Here, we extended the in vitro assays to show the pro-angiogenic effects of d-hDPSCs, such as enhanced endothelial cell proliferation, migration and differentiation. In addition, for the first time we evaluated the performance of d-hDPSCs in an in vivo rat model of PNI. Eight weeks after transplantation of NeuraWrap conduits filled with engineered neural tissue (EngNT) containing aligned d-hDPSCs in 15-mm rat sciatic nerve defects, immunohistochemistry and ultrastructural analysis revealed ingrowing neurites, myelinated nerve fibres and blood vessels along the construct. Although further research is required to optimize the delivery of this EngNT, our findings suggest that d-hDPSCs are able to exert a positive effect in the regeneration of nerve tissue in vivo. Copyright (c) 2017 John Wiley & Sons, Ltd. | Notes: | [Sanen, Kathleen; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, Biophys Grp, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Martens, Wendy; Lambrichts, Ivo] Hasselt Univ, Biomed Res Inst, Morphol Grp, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Georgiou, Melanie] UCL, Adv Ctr Biochem Engn, Bernard Katz Bldg,Gordon St, London WC1H 0AH, England. [Phillips, James] UCL, UCL Eastman Dent Inst, Biomat & Tissue Engn, 256 Grays Inn Rd, London WC1X 8LD, England. | Keywords: | peripheral nerve injury; human dental pulp stem cells; Schwann cells; angiogenesis; neurite regeneration; nerve tissue engineering;peripheral nerve injury; human dental pulp stem cells; Schwann cells; angiogenesis; neurite regeneration; nerve tissue engineering | Document URI: | http://hdl.handle.net/1942/26352 | ISSN: | 1932-6254 | e-ISSN: | 1932-7005 | DOI: | 10.1002/term.2249 | ISI #: | 000418255700007 | Rights: | Copyright © 2017 John Wiley & Sons, Ltd. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
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