Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/26357
Title: Dihydrofolate Reductase/Thymidylate Synthase Fine-Tunes the Folate Status and Controls Redox Homeostasis in Plants
Other Titles: DHFR-TS regulates the folate pool and redox state
Authors: Gorelova, Vera
De Lepeleire, Jolien
Van Daele, Jeroen
Pluim, Dick
Mei, Coline
Cuypers, Ann 
Leroux, Olivier
Rebeille, Fabrice
Schellens, Jan H. M.
Blancquaert, Dieter
Stove, Christophe P.
Van Der Straeten, Dominique
Issue Date: 2017
Publisher: AMER SOC PLANT BIOLOGISTS
Source: PLANT CELL, 29(11), p. 2831-2853
Abstract: Folates (B9 vitamins) are essential cofactors in one-carbon metabolism. Since C1 transfer reactions are involved in synthesis of nucleic acids, proteins, lipids, and other biomolecules, as well as in epigenetic control, folates are vital for all living organisms. This work presents a complete study of a plant DHFR-TS (dihydrofolate reductase-thymidylate synthase) gene family that implements the penultimate step in folate biosynthesis. We demonstrate that one of the DHFR-TS isoforms (DHFRTS3) operates as an inhibitor of its two homologs, thus regulating DHFR and TS activities and, as a consequence, folate abundance. In addition, a novel function of folate metabolism in plants is proposed, i.e., maintenance of the redox balance by contributing to NADPH production through the reaction catalyzed by methylenetetrahydrofolate dehydrogenase, thus allowing plants to cope with oxidative stress.
Notes: [Gorelova, Vera; De Lepeleire, Jolien; Blancquaert, Dieter; Van Der Straeten, Dominique] Univ Ghent, Dept Biol, Lab Funct Plant Biol, B-9000 Ghent, Belgium. [Van Daele, Jeroen; Stove, Christophe P.] Univ Ghent, Lab Toxicol, B-9000 Ghent, Belgium. [Pluim, Dick; Schellens, Jan H. M.] Netherlands Canc Inst, Lab Mol Pathol, NL-1066 CX Amsterdam, Netherlands. [Mei, Coline; Rebeille, Fabrice] Univ Joseph Fourier Grenoble 1, CNRS, Biosci & Biotechnol Inst Grenoble,UMR168, CEA,INRA,Lab Physiol Cellularie Vegetale,CEA Gren, F-38054 Grenoble 9, France. [Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Leroux, Olivier] Univ Ghent, Dept Biol, B-9000 Ghent, Belgium.
Document URI: http://hdl.handle.net/1942/26357
ISSN: 1040-4651
e-ISSN: 1532-298X
DOI: 10.1105/tpc.17.00433
ISI #: 000417643700015
Rights: ©2017 American Society of Plant Biologists. All Rights Reserved
Category: A1
Type: Journal Contribution
Validations: ecoom 2019
Appears in Collections:Research publications

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