Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/26365
Full metadata record
DC FieldValueLanguage
dc.contributor.authorHammer, Anna-
dc.contributor.authorSchliep, Anne-
dc.contributor.authorJoerg, Stefanie-
dc.contributor.authorHaghikia, Aiden-
dc.contributor.authorGold, Ralf-
dc.contributor.authorKLEINEWIETFELD, Markus-
dc.contributor.authorMueller, Dominik N.-
dc.contributor.authorLinker, Ralf A.-
dc.date.accessioned2018-07-18T07:55:46Z-
dc.date.available2018-07-18T07:55:46Z-
dc.date.issued2017-
dc.identifier.citationJOURNAL OF NEUROINFLAMMATION, 14 (Art N° 184)-
dc.identifier.issn1742-2094-
dc.identifier.urihttp://hdl.handle.net/1942/26365-
dc.description.abstractBackground: There has been a marked increase in the incidence of autoimmune diseases like multiple sclerosis (MS) in the last decades which is most likely driven by a change in environmental factors. Here, growing evidence suggests that ingredients of a Western diet like high intake of sodium chloride (NaCl) or saturated fatty acids may impact systemic immune responses, thus increasing disease susceptibility. Recently, we have shown that high dietary salt or long-chain fatty acid (LCFA) intake indeed aggravates T helper (Th) cell responses and neuroinflammation. Methods: Naive CD4(+) T cells were treated with an excess of 40 mM NaCl and/or 250 mu M lauric acid (LA) in vitro to analyze effects on Th cell differentiation, cytokine secretion, and gene expression. We employed ex vivo analyses of the model disease murine experimental autoimmune encephalomyelitis (EAE) to investigate whether salt and LCFA may affect disease severity and T cell activation in vivo. Results: LCFA, like LA, together with NaCl enhance the differentiation of Th1 and Th17 cells as well as proinflammatory cytokine and gene expression in vitro. In cell culture, we observed an additive effect of LA and hypertonic extracellular NaCl (NaCl + LA) in Th17 differentiation assays as well as on IL-17, GM-CSF, and IL-2 gene expression. In contrast, NaCl + LA reduced Th2 frequencies. We employed EAE as a model of Th1/Th17 cell-mediated autoimmunity and show that the combination of a NaCl-and LA-rich diet aggravated the disease course and increased T cell infiltration into the central nervous system (CNS) to the same extent as dietary NaCl. Conclusions: Our findings demonstrate a partially additive effect of NaCl and LA on Th cell polarization in vitro and on Th cell responses in autoimmune neuroinflammation. These data may help to better understand the pathophysiology of autoimmune diseases such as MS.-
dc.description.sponsorshipEuropean Research Council (ERC) under the European Union [640116]; government of Flanders, Belgium; Research Foundation Flanders (FWO), Belgium-
dc.language.isoen-
dc.publisherBIOMED CENTRAL LTD-
dc.subject.otherT helper cell:-
dc.subject.otherDiet-
dc.subject.otherSalt-
dc.subject.otherSaturated fatty acids-
dc.subject.otherMultiple sclerosis-
dc.subject.otherEAE-
dc.subject.otherInflammation-
dc.titleImpact of combined sodium chloride and saturated long-chain fatty acid challenge on the differentiation of T helper cells in neuroinflammation-
dc.typeJournal Contribution-
dc.identifier.volume14-
local.format.pages9-
local.bibliographicCitation.jcatA1-
dc.description.notes[Hammer, Anna; Schliep, Anne; Joerg, Stefanie; Linker, Ralf A.] Friedrich Alexander Univ Erlangen Nurnberg, Univ Hosp Erlangen, Dept Neurol, Schwabachanlage 6, D-91054 Erlangen, Germany. [Haghikia, Aiden; Gold, Ralf] Ruhr Univ Bochum, Dept Neurol, D-44801 Bochum, Germany. [Kleinewietfeld, Markus] Hasselt Univ, VIB Lab Translat Immunomodulat, VIB Ctr Inflammat Res IRC, BIOMED, B-3590 Diepenbeek, Belgium. [Mueller, Dominik N.] Expt & Clin Res Ctr, D-13125 Berlin, Germany. [Mueller, Dominik N.] Helmholtz Assoc, Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany. [Mueller, Dominik N.] BIH, Berlin, Germany.-
dc.description.notesLinker, RA (reprint author), Friedrich Alexander Univ Erlangen Nurnberg, Univ Hosp Erlangen, Dept Neurol, Schwabachanlage 6, D-91054 Erlangen, Germany.ralf.linker@uk-erlangen.de-
local.publisher.placeLONDON-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.bibliographicCitation.artnr184-
local.classdsPublValOverrule/author_version_not_expected-
local.type.programmehorizonEurope-
dc.identifier.doi10.1186/s12974-017-0954-y-
dc.identifier.isi000410372100001-
local.uhasselt.internationalyes-
local.relation.horizonEurope640116-
item.validationecoom 2018-
item.fulltextWith Fulltext-
item.fullcitationHammer, Anna; Schliep, Anne; Joerg, Stefanie; Haghikia, Aiden; Gold, Ralf; KLEINEWIETFELD, Markus; Mueller, Dominik N. & Linker, Ralf A. (2017) Impact of combined sodium chloride and saturated long-chain fatty acid challenge on the differentiation of T helper cells in neuroinflammation. In: JOURNAL OF NEUROINFLAMMATION, 14 (Art N° 184).-
item.accessRightsOpen Access-
item.contributorHammer, Anna-
item.contributorSchliep, Anne-
item.contributorJoerg, Stefanie-
item.contributorHaghikia, Aiden-
item.contributorGold, Ralf-
item.contributorKLEINEWIETFELD, Markus-
item.contributorMueller, Dominik N.-
item.contributorLinker, Ralf A.-
crisitem.journal.issn1742-2094-
crisitem.journal.eissn1742-2094-
Appears in Collections:Research publications
Files in This Item:
File Description SizeFormat 
s12974-017-0954-y.pdfPublished version910.23 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

16
checked on Sep 2, 2020

WEB OF SCIENCETM
Citations

34
checked on Jul 11, 2024

Page view(s)

64
checked on Sep 7, 2022

Download(s)

112
checked on Sep 7, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.