Nickel-resistance-based minitransposons: New tools for genetic manipulation of environmental bacteria

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2671

Title:	Nickel-resistance-based minitransposons: New tools for genetic manipulation of environmental bacteria
Authors:	Taghavi, S Delanghe, H LODEWYCKX, CINDY Mergeay, M. VAN DER LELIE, Daniel
Issue Date:	2001
Publisher:	AMER SOC MICROBIOLOGY
Source:	APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 67(2). p. 1015-1019
Abstract:	The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre mini-transposons in members of the alpha, beta, and gamma subclasses of the Proteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.
Notes:	Vlaasme Instelling Technol Onderzoek, Environm Technol Ctr, B-2400 Mol, Belgium. Limburgs Univ Ctr, B-3590 Diepenbeek, Belgium.van der Lelie, D, Vlaasme Instelling Technol Onderzoek, Environm Technol Ctr, Boeretang 200, B-2400 Mol, Belgium.
Document URI:	http://hdl.handle.net/1942/2671
ISSN:	0099-2240
e-ISSN:	1098-5336
DOI:	10.1128/AEM.67.2.1015-1019.2001
ISI #:	000166844600076
Category:	A1
Type:	Journal Contribution
Validations:	ecoom 2002
Appears in Collections:	Research publications

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