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http://hdl.handle.net/1942/27237
Title: | Quantitative Peptidomics with Isotopic and Isobaric Tags | Authors: | Boonen, Kurt De Haes, Wouter VAN HOUTVEN, Joris VERDONCK, Rik Baggerman, Geert VALKENBORG, Dirk Schoofs, Liliane |
Issue Date: | 2018 | Publisher: | Humana Press | Source: | Schrader, Michael; Fricker, Lloyd (Ed.). Peptidomics: Methods and Strategies, Humana Press, p. 141-159 | Series/Report: | Methods in Molecular Biology | Series/Report no.: | 1719 | Abstract: | In differential peptidomics, peptide profiles are compared between biological samples and the resulting expression levels are correlated to a phenotype of interest. This, in turn, allows us insight into how peptides may affect the phenotype of interest. In quantitative differential peptidomics, both label-based and label-free techniques are often employed. Label-based techniques have several advantages over label-free methods, primarily that labels allow for various samples to be pooled prior to liquid chromatography-mass spectrometry (LC-MS) analysis, reducing between-run variation. Here, we detail a method for performing quantitative peptidomics using stable amine-binding isotopic and isobaric tags. | Keywords: | peptidomics; isotopic tags; isobaric tags; mass spectrometry; protocols | Document URI: | http://hdl.handle.net/1942/27237 | ISBN: | 9781493975372 | DOI: | 10.1007/978-1-4939-7537-2_9 | Rights: | Springer Science+Business Media, LLC 2018 | Category: | B2 | Type: | Book Section | Validations: | vabb 2020 |
Appears in Collections: | Research publications |
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