Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/27237
Title: Quantitative Peptidomics with Isotopic and Isobaric Tags
Authors: Boonen, Kurt
De Haes, Wouter
VAN HOUTVEN, Joris 
Verdonck, Rik
Baggerman, Geert
VALKENBORG, Dirk 
Schoofs, Liliane
Issue Date: 2018
Publisher: Humana Press
Source: Schrader, Michael; Fricker, Lloyd (Ed.). Peptidomics: Methods and Strategies, Humana Press, p. 141-159
Series/Report: Methods in Molecular Biology
Series/Report no.: 1719
Abstract: In differential peptidomics, peptide profiles are compared between biological samples and the resulting expression levels are correlated to a phenotype of interest. This, in turn, allows us insight into how peptides may affect the phenotype of interest. In quantitative differential peptidomics, both label-based and label-free techniques are often employed. Label-based techniques have several advantages over label-free methods, primarily that labels allow for various samples to be pooled prior to liquid chromatography-mass spectrometry (LC-MS) analysis, reducing between-run variation. Here, we detail a method for performing quantitative peptidomics using stable amine-binding isotopic and isobaric tags.
Keywords: peptidomics; isotopic tags; isobaric tags; mass spectrometry; protocols
Document URI: http://hdl.handle.net/1942/27237
ISBN: 9781493975372
DOI: 10.1007/978-1-4939-7537-2_9
Rights: Springer Science+Business Media, LLC 2018
Category: B2
Type: Book Section
Validations: vabb 2020
Appears in Collections:Research publications

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