Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/27260
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dc.contributor.authorBorrenberghs, D.-
dc.contributor.authorZurnic, I.-
dc.contributor.authorDe Wit, F.-
dc.contributor.authorAcke, A.-
dc.contributor.authorDirix, L.-
dc.contributor.authorCereseto, A.-
dc.contributor.authorDebyser, Z.-
dc.contributor.authorHENDRIX, Jelle-
dc.date.accessioned2018-10-30T11:26:38Z-
dc.date.available2018-10-30T11:26:38Z-
dc.date.issued2018-
dc.identifier.citationNUCLEIC ACIDS RESEARCH, 47 (3), p. 1195-1210-
dc.identifier.issn0305-1048-
dc.identifier.urihttp://hdl.handle.net/1942/27260-
dc.description.abstractThe Moloney murine leukemia virus (MLV) is a prototype gammaretrovirus requiring nuclear disassembly before DNA integration. In the nucleus, integration site selection towards promoter/enhancer elements is mediated by the host factor bromo- and extraterminal domain (BET) proteins (bromodomain (Brd) proteins 2, 3 and 4). MLV-based retroviral vectors are used in gene therapy trials. In some trials leukemia occurred through integration of the MLV vector in close proximity to cellular oncogenes. BET-mediated integration is poorly understood and the nature of integrase oligomers heavily debated. Here, we created wild-type infectious MLV vectors natively incorporating fluorescent labeled IN and performed single-molecule intensity and Förster resonance energy transfer experiments. The nuclear localization of the MLV pre-integration complex neither altered the IN content, nor its quaternary structure. Instead, BET-mediated interaction of the MLV intasome with chromatin in the post-mitotic nucleus reshaped its quaternary structure.-
dc.description.sponsorshipFWO [G0B49.15]; KU Leuven Research Council [OT; OT/13/098]; HIV-ERA EURECA [IWT-SBO-EURECA]; KU Leuven IDO program [IDO/12/008]; Belgian IAP Belvir and the Methusalem program [CASAS METH/08/04]. Funding for open access charge: project finance.-
dc.language.isoen-
dc.titlePost-mitotic BET-induced reshaping of integrase quaternary structure supports wild-type MLV integration-
dc.typeJournal Contribution-
dc.identifier.epage1210-
dc.identifier.issue3-
dc.identifier.spage1195-
dc.identifier.volume47-
local.bibliographicCitation.jcatA1-
dc.description.notesHendrix, J (reprint author), Katholieke Univ Leuven, Dept Chem, Lab Photochem & Spect, Celestijnenlaan 200F, B-3001 Leuven, Belgium. Hasselt Univ, Adv Opt Microscopy Ctr, Dynam Bioimaging Lab, Agoralaan C, B-3590 Diepenbeek, Belgium. Hasselt Univ, Biomed Res Inst BIOMED, Agoralaan C, B-3590 Diepenbeek, Belgium Debyser, Z (reprint author), Katholieke Univ Leuven, Dept Pharmaceut & Pharmacol Sci, Lab Mol Virol & Gene Therapy, Kapucijnenvoer 33, B-3001 Leuven, Flanders, Belgium. zeger.debyser@kuleuven.be; jelle.hendrix@uhasselt.be-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1093/nar/gky1157-
dc.identifier.isi000467960500017-
item.fullcitationBorrenberghs, D.; Zurnic, I.; De Wit, F.; Acke, A.; Dirix, L.; Cereseto, A.; Debyser, Z. & HENDRIX, Jelle (2018) Post-mitotic BET-induced reshaping of integrase quaternary structure supports wild-type MLV integration. In: NUCLEIC ACIDS RESEARCH, 47 (3), p. 1195-1210.-
item.validationecoom 2020-
item.accessRightsOpen Access-
item.fulltextWith Fulltext-
item.contributorBorrenberghs, D.-
item.contributorZurnic, I.-
item.contributorDe Wit, F.-
item.contributorAcke, A.-
item.contributorDirix, L.-
item.contributorCereseto, A.-
item.contributorDebyser, Z.-
item.contributorHENDRIX, Jelle-
crisitem.journal.issn0305-1048-
crisitem.journal.eissn1362-4962-
Appears in Collections:Research publications
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