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http://hdl.handle.net/1942/27433
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DC Field | Value | Language |
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dc.contributor.author | Koppen, Gudrun | - |
dc.contributor.author | De Prins, Sofie | - |
dc.contributor.author | Jacobs, An | - |
dc.contributor.author | Nelen, Vera | - |
dc.contributor.author | Schoeters, Greet | - |
dc.contributor.author | LANGIE, Sabine | - |
dc.date.accessioned | 2018-11-19T10:47:01Z | - |
dc.date.available | 2018-11-19T10:47:01Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | MUTAGENESIS, 33(1), p. 41-47 | - |
dc.identifier.issn | 0267-8357 | - |
dc.identifier.uri | http://hdl.handle.net/1942/27433 | - |
dc.description.abstract | The comet assay is often applied in human biomonitoring. Most of the time the assay is performed with isolated peripheral blood mononuclear cells (PBMC). However, using whole blood instead of isolated cells reduces processing time, and only 20 mu l is sufficient for analysis. In this study, a cryopreservation protocol for human whole blood for application in the comet assay was optimised by removing excess plasma before adding freezing medium. Cryopreservation of whole blood samples (n = 30) did not increase the detected level of strand breaks and formamidopyrimidine DNA glycosylase (FPG)-sensitive sites. Although there was no significant correlation with breaks measured in fresh whole blood, strand breaks detected in frozen whole blood were significantly correlated with breaks measured in frozen PBMC (Pearson correlation r = 0.54, P < 0.01). This correlation was however not observed for FPG-sensitive sites. Since we do not yet know the full extent to which cryopreservation might influence the blood cell population, care should be taken to ensure a similar cell type and storage conditions for all samples in one study. | - |
dc.description.sponsorship | This study was partially financed by the Flemish government (Environment, Nature and Energy Department, Environment & Health unit) and the EU-FP 6 INTARESE (Integrated Assessment of Health Risks of Environmental Stressors in Europe) project. S.L. is the beneficiary of a postdoctoral fellowship (12L5216N; http://www.fwo.be/) provided by The Research Foundation Flanders (FWO) and the Flemish Institute of Technological Research (VITO). We kindly thank Dr Gunnar Brunborg (Norwegian Institute of Public Health, Oslo, Norway) for providing the FPG enzyme and the Ro12-9786 component. | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.rights | The Author(s) 2017. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com | - |
dc.subject.other | comet assay; cryopreservation; dna; whole blood; peripheral blood mononuclear cell | - |
dc.title | The comet assay in human biomonitoring: cryopreservation of whole blood and comparison with isolated mononuclear cells | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 47 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 41 | - |
dc.identifier.volume | 33 | - |
local.format.pages | 7 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Koppen, Gudrun; De Prins, Sofie; Jacobs, An; Schoeters, Greet; Langie, Sabine A. S.] Flemish Inst Technol Res VITO, Hlth Unit, Mol, Belgium. [De Prins, Sofie; Schoeters, Greet] VITO Sustainable Hlth, Mol, Belgium. [Nelen, Vera] Prov Inst Hyg, Environm & Hlth Unit, Antwerp, Belgium. [Schoeters, Greet] Univ Southern Denmark, Inst Publ Hlth, Dept Environm Med, Odense, Denmark. [Langie, Sabine A. S.] Hasselt Univ, Fac Sci, Diepenbeek, Belgium. | - |
local.publisher.place | GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1093/mutage/gex034 | - |
dc.identifier.isi | 000426079100006 | - |
dc.identifier.eissn | 1464-3804 | - |
local.uhasselt.international | yes | - |
item.fulltext | With Fulltext | - |
item.fullcitation | Koppen, Gudrun; De Prins, Sofie; Jacobs, An; Nelen, Vera; Schoeters, Greet & LANGIE, Sabine (2018) The comet assay in human biomonitoring: cryopreservation of whole blood and comparison with isolated mononuclear cells. In: MUTAGENESIS, 33(1), p. 41-47. | - |
item.accessRights | Restricted Access | - |
item.contributor | Koppen, Gudrun | - |
item.contributor | De Prins, Sofie | - |
item.contributor | Jacobs, An | - |
item.contributor | Nelen, Vera | - |
item.contributor | Schoeters, Greet | - |
item.contributor | LANGIE, Sabine | - |
crisitem.journal.issn | 0267-8357 | - |
crisitem.journal.eissn | 1464-3804 | - |
Appears in Collections: | Research publications |
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gex034.pdf Restricted Access | Published version | 558.23 kB | Adobe PDF | View/Open Request a copy |
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