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http://hdl.handle.net/1942/27697
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DC Field | Value | Language |
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dc.contributor.author | KELLENS, Evelien | - |
dc.contributor.author | BOVE, Hannelore | - |
dc.contributor.author | VANDENRYT, Thijs | - |
dc.contributor.author | LAMBRICHTS, Jeroen | - |
dc.contributor.author | Dekens, Jolien | - |
dc.contributor.author | DRIJKONINGEN, Sien | - |
dc.contributor.author | D'HAEN, Jan | - |
dc.contributor.author | DE CEUNINCK, Ward | - |
dc.contributor.author | THOELEN, Ronald | - |
dc.contributor.author | JUNKERS, Tanja | - |
dc.contributor.author | HAENEN, Ken | - |
dc.contributor.author | ETHIRAJAN, Anitha | - |
dc.date.accessioned | 2019-02-11T11:13:16Z | - |
dc.date.available | 2019-02-11T11:13:16Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, 118, p. 58-65 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/1942/27697 | - |
dc.description.abstract | Molecularly imprinted polymers (MIPs) can selectively bind target molecules and can therefore be advantageously used as a low-cost and robust alternative to replace fragile and expensive natural receptors. Yet, one major challenge in using MIPs for sensor development is the lack of simple and cost-effective techniques that allow firm fixation as well as controllable and consistent receptor material distribution on the sensor substrate. In this work, a convenient method is presented wherein microfluidic systems in conjunction with in situ photo-polymerization on functionalized diamond substrates are used. This novel strategy is simple, efficient, low-cost and less time consuming. Moreover, the approach ensures a tunable and consistent MIP material amount and distribution between different sensor substrates and thus a controllable active sensing surface. The obtained patterned MIP structures are successfully tested as a selective sensor platform to detect physiological concentrations of the hormone disruptor testosterone in buffer, urine and saliva using electrochemical impedance spectroscopy. The highest added testosterone concentration (500 nM) in buffer resulted in an impedance signal of 10.03 +/- 0.19% and the lowest concentration (0.5 nM) led to a measurable signal of 1.8 +/- 0.15% for the MIPs. With a detection limit of 0.5 nM, the MIP signals exhibited good linearity between a 0.5 nM and 20 nM concentration range. Apart from the excellent and selective recognition offered by these MIP structures, they are also stable during and after the dynamic sensor measurements. Additionally, the MIPs can be easily regenerated by a simple washing procedure and are successfully tested for their reusability. | - |
dc.description.sponsorship | E.K. and H.B. contributed equally to this work. E.K. gratefully acknowledges the financial support by the Agency for Innovation by Science and Technology (IWT, 121354), Belgium. H.B. is an FWO (Research Foundation Flanders, 11ZB115N) doctoral fellow. K.H. acknowledges the financial support provided by the Methusalem "NANO" Network. The authors also thank additional financial support from UHasselt BOF Funds. The authors gratefully acknowledge technical support by B. Ruttens, H. Pellaers, C. Willems, and J. Baccus. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject.other | Microfluidics; Molecularly imprinted polymers; Biosensors and Bodyfluids | - |
dc.subject.other | Microfluidics; Molecularly imprinted polymers; Biosensors and Body fluids | - |
dc.title | Micro-patterned molecularly imprinted polymer structures on functionalized diamond-coated substrates for testosterone detection | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 65 | - |
dc.identifier.spage | 58 | - |
dc.identifier.volume | 118 | - |
local.format.pages | 8 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Kellens, Evelien; Bove, Hannelore; Vandenryt, Thijs; Lambrichts, Jeroen; Dekens, Jolien; Drijkoningen, Sien; D'Haen, Jan; De Ceuninck, Ward; Thoelen, Ronald; Junkers, Tanja; Haenen, Ken; Ethirajan, Anitha] Hasselt Univ, Inst Mat Res IMO, Wetenschapspk 1 & Agoralaan D, B-3590 Diepenbeek, Belgium. [Kellens, Evelien; Vandenryt, Thijs; Lambrichts, Jeroen; Drijkoningen, Sien; D'Haen, Jan; De Ceuninck, Ward; Thoelen, Ronald; Haenen, Ken; Ethirajan, Anitha] IMEC VZW, IMOMEC, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Bove, Hannelore] Hasselt Univ, Biomed Res Inst BIOMED, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Junkers, Tanja] Monash Univ, Sch Chem, 19 Rainforest Walk, Clayton, Vic 3800, Australia. | - |
local.publisher.place | OXFORD | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.bios.2018.07.032 | - |
dc.identifier.isi | 000444358000009 | - |
item.validation | ecoom 2019 | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
item.fullcitation | KELLENS, Evelien; BOVE, Hannelore; VANDENRYT, Thijs; LAMBRICHTS, Jeroen; Dekens, Jolien; DRIJKONINGEN, Sien; D'HAEN, Jan; DE CEUNINCK, Ward; THOELEN, Ronald; JUNKERS, Tanja; HAENEN, Ken & ETHIRAJAN, Anitha (2018) Micro-patterned molecularly imprinted polymer structures on functionalized diamond-coated substrates for testosterone detection. In: BIOSENSORS & BIOELECTRONICS, 118, p. 58-65. | - |
item.contributor | KELLENS, Evelien | - |
item.contributor | BOVE, Hannelore | - |
item.contributor | VANDENRYT, Thijs | - |
item.contributor | LAMBRICHTS, Jeroen | - |
item.contributor | Dekens, Jolien | - |
item.contributor | DRIJKONINGEN, Sien | - |
item.contributor | D'HAEN, Jan | - |
item.contributor | DE CEUNINCK, Ward | - |
item.contributor | THOELEN, Ronald | - |
item.contributor | JUNKERS, Tanja | - |
item.contributor | HAENEN, Ken | - |
item.contributor | ETHIRAJAN, Anitha | - |
crisitem.journal.issn | 0956-5663 | - |
crisitem.journal.eissn | 1873-4235 | - |
Appears in Collections: | Research publications |
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kellens 1.pdf Restricted Access | Published version | 831.28 kB | Adobe PDF | View/Open Request a copy |
Manuscript_AfterRevision.pdf | Peer-reviewed author version | 1.64 MB | Adobe PDF | View/Open |
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