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http://hdl.handle.net/1942/27857
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DC Field | Value | Language |
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dc.contributor.author | BOVE, Hannelore | - |
dc.contributor.author | DEVOGHT, Jens | - |
dc.contributor.author | RASKING, Leen | - |
dc.contributor.author | PETERS, Martijn | - |
dc.contributor.author | SLENDERS, Eli | - |
dc.contributor.author | Roeffaers, Maarten | - |
dc.contributor.author | Jorge-Penas, Alvaro | - |
dc.contributor.author | Van Oosterwyck, Hans | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.date.accessioned | 2019-03-06T14:38:57Z | - |
dc.date.available | 2019-03-06T14:38:57Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | JOURNAL OF NANOBIOTECHNOLOGY, 16 (Art N° 82) | - |
dc.identifier.issn | 1477-3155 | - |
dc.identifier.uri | http://hdl.handle.net/1942/27857 | - |
dc.description.abstract | Background The continuously growing human exposure to combustion-derived particles (CDPs) drives in depth investigation of the involved complex toxicological mechanisms of those particles. The current study evaluated the hypothesis that CDPs could affect cell-induced remodeling of the extracellular matrix due to their underlying toxicological mechanisms. The effects of two ultrafine and one fine form of CDPs on human lung fibroblasts (MRC-5 cell line) were investigated, both in 2D cell culture and in 3D collagen type I hydrogels. A multi-parametric analysis was employed.ResultsIn vitro dynamic 3D analysis of collagen matrices showed that matrix displacement fields induced by human lung fibroblasts are disturbed when exposed to carbonaceous particles, resulting in inhibition of matrix remodeling. In depth analysis using general toxicological assays revealed that a plausible explanation comprises a cascade of numerous detrimental effects evoked by the carbon particles, including oxidative stress, mitochondrial damage and energy storage depletion. Also, ultrafine particles revealed stronger toxicological and inhibitory effects compared to their larger counterparts. The inhibitory effects can be almost fully restored when treating the impaired cells with antioxidants like vitamin C.ConclusionsThe unraveled in vitro pathway, by which ultrafine particles alter the fibroblasts' vital role of matrix remodeling, extends our knowledge about the contribution of these biologically active particles in impaired lung tissue repair mechanisms, and development and exacerbation of chronic lung diseases. The new insights may even pave the way to precautionary actions. The results provide justification for toxicological assessments to include mechanism-linked assays besides the traditional in vitro toxicological screening assays. | - |
dc.description.sponsorship | This research was supported by the Interuniversity Attraction Poles Program (P7/05) initiated by the Belgian Science Policy Office. H.B. acknowledges funding from Research Foundation Flanders (Fonds Wetenschappelijk Onderzoek, FWO) for the doctoral fellowship 11ZB115N. The authors also thank FWO for the research Grant G.0821.13. M.R. acknowledges financial support from the KU Leuven Research Fund (C14/15/053) and FWO (AKUL/15/15 - G0H0816N). M.A. thanks the Province of Limburg (Belgium) for the financial support within the tUL IMPULS FASE II program, allowing for the upgrading of the laser source used in this work. H.V.O. acknowledges funding from the European Research Council under the European Union's Seventh Framework Program (FP7/2007-2013)/ERC Grant Agreement no 308223). | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.rights | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | - |
dc.subject.other | Combustion-derived particles; In vitro toxicology; Human lung fibroblasts; Hazard assessment; Matrix remodeling inhibition | - |
dc.subject.other | Combustion-derived particles; In vitro toxicology; Human lung fbroblasts; Hazard assessment; Matrix remodeling inhibition | - |
dc.title | Combustion-derived particles inhibit in vitro human lung fibroblast-mediated matrix remodeling | - |
dc.type | Journal Contribution | - |
dc.identifier.volume | 16 | - |
local.format.pages | 12 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Bove, Hannelore; Devoght, Jens; Rasking, Leentje; Slenders, Eli; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, Agoralaan Bldg C, Diepenbeek, Belgium. [Bove, Hannelore; Roeffaers, Maarten] Katholieke Univ Leuven, Ctr Surface Chem & Catalysis, Celestijnenlaan 200F, Louvain, Belgium. [Peters, Martijn] Hasselt Univ, Inst Mat Res, Agoralaan Bldg D, Diepenbeek, Belgium. [Jorge-Penas, Alvaro; Van Oosterwyck, Hans] Katholieke Univ Leuven, Dept Mech Engn, Celestijnenlaan 300C,Box 2419, Louvain, Belgium. [Van Oosterwyck, Hans] Katholieke Univ Leuven, Div Skeletal Tissue Engn, Prometheus, Louvain, Belgium. | - |
local.publisher.place | LONDON | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 82 | - |
dc.identifier.doi | 10.1186/s12951-018-0410-x | - |
dc.identifier.isi | 000448504400001 | - |
item.validation | ecoom 2019 | - |
item.contributor | BOVE, Hannelore | - |
item.contributor | DEVOGHT, Jens | - |
item.contributor | RASKING, Leen | - |
item.contributor | PETERS, Martijn | - |
item.contributor | SLENDERS, Eli | - |
item.contributor | Roeffaers, Maarten | - |
item.contributor | Jorge-Penas, Alvaro | - |
item.contributor | Van Oosterwyck, Hans | - |
item.contributor | AMELOOT, Marcel | - |
item.accessRights | Open Access | - |
item.fullcitation | BOVE, Hannelore; DEVOGHT, Jens; RASKING, Leen; PETERS, Martijn; SLENDERS, Eli; Roeffaers, Maarten; Jorge-Penas, Alvaro; Van Oosterwyck, Hans & AMELOOT, Marcel (2018) Combustion-derived particles inhibit in vitro human lung fibroblast-mediated matrix remodeling. In: JOURNAL OF NANOBIOTECHNOLOGY, 16 (Art N° 82). | - |
item.fulltext | With Fulltext | - |
crisitem.journal.eissn | 1477-3155 | - |
Appears in Collections: | Research publications |
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bove 1.pdf | Published version | 1.56 MB | Adobe PDF | View/Open |
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