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Title: | Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells | Authors: | SLENDERS, Eli Boye, Hannelore Urbain, Mathias Mugnier, Yannick Sonay, Ali Yasin Pantazis, Periklis Bonacina, Luigi Berghe, Pieter Vanden vandeVen, Martin AMELOOT, Marcel |
Issue Date: | 2018 | Publisher: | AMER CHEMICAL SOC | Source: | JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 9(20), p. 6112-6118 | Abstract: | The absence of photobleaching, blinking, and saturation combined with a high contrast provides unique advantages of higher-harmonic generating nanoparticles over fluorescent probes, allowing for prolonged correlation spectroscopy studies. We apply the coherent intensity fluctuation model to study the mobility of second harmonic generating nanoparticles. A concise protocol is presented for quantifying the diffusion coefficient from a single spectroscopy measurement without the need for separate point-spread-function calibrations. The technique's applicability is illustrated on nominally 56 nm LiNbO3 nanoparticles. We perform label-free raster image correlation spectroscopy imaging in aqueous suspension and spatiotemporal image correlation spectroscopy in A549 human lung carcinoma cells. In good agreement with the expected theoretical result, the measured diffusion coefficient in water at room temperature is (7.5 +/- 0.3) mu m(2)/s. The diffusion coefficient in the cells is more than 10(3) times lower and heterogeneous, with an average of (3.7 +/- 1.5) X 10(-3) mu m(2)/s. | Notes: | [Slenders, Eli; Boye, Hannelore; vandeVen, Martin; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst BIOMED, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Urbain, Mathias; Mugnier, Yannick] Univ Savoie Mt Blanc, SYMME, F-74000 Annecy, France. [Sonay, Ali Yasin; Pantazis, Periklis] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, Mattenstr 26, CH-4058 Basel, Switzerland. [Pantazis, Periklis] Imperial Coll London, Dept Bioengn, South Kensington Campus, London SW7 2AZ, England. [Bonacina, Luigi] Univ Geneva, Dept Appl Phys, Chemin Pinchat 22, CH-1211 Geneva, Switzerland. [Berghe, Pieter Vanden] Univ Leuven, Lab Enter Neurosci LENS, TARGID, Herestr 49, B-3000 Leuven, Belgium. | Document URI: | http://hdl.handle.net/1942/28059 | ISSN: | 1948-7185 | DOI: | 10.1021/acs.jpclett.8b02686 | ISI #: | 000448751700031 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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slenders 1.pdf Restricted Access | Published version | 2.01 MB | Adobe PDF | View/Open Request a copy |
author version.pdf | Peer-reviewed author version | 2.81 MB | Adobe PDF | View/Open |
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