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http://hdl.handle.net/1942/28230
Title: | Human papillomavirus detection in urine: Effect of a first-void urine collection device and timing of collection | Authors: | Pattyn, Jade Van Keer, Severien Biesmans, Samantha Ieven, Margareta Vanderborght, Charlotte Beyers, Koen Vankerckhoven, Vanessa BRUYNDONCKX, Robin Van Damme, Pierre Vorsters, Alex |
Issue Date: | 2019 | Publisher: | ELSEVIER SCIENCE BV | Source: | JOURNAL OF VIROLOGICAL METHODS, 264, p. 23-30 | Abstract: | Great interest has been directed towards the use of first-void (FV) urine as a liquid biopsy for high-risk HPV DNA testing. The aim of this study was to investigate the potential effect of a first generation FV urine collection device on the detection of HPV DNA and to assess if the concentration of HPV DNA varies between FV urine collected in the morning and those collected later during the day. In this prospective cohort study, 33 self-reported HPV-positive women participated. An FV urine sample was collected by these women in the morning (first urine of the day) and another sample was collected later that day for four consecutive days using two different collection methods; i.e., the Colli-Pee (R) and a standard urine cup. Samples were collected at home and returned at ambient temperature to the laboratory by postal mail. HPV DNA testing was conducted with the Riatol qPCR HPV genotyping assay. Based on the combined generalized linear mixed model used, there was no significant impact of the timing of collection (morning versus later during the day) on copies of HPV DNA, whereas Colli-Pee (R) collected samples show higher HPV concentrations than cup collected samples. However, at high concentrations of hDNA, the benefit of the Colli-Pee (R) disappeared. | Notes: | [Pattyn, Jade; Van Keer, Severien; Biesmans, Samantha; Ieven, Margareta; Vanderborght, Charlotte; Vankerckhoven, Vanessa; Van Damme, Pierre; Vorsters, Alex] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, CEV, Fac Med & Hlth Sci, Antwerp, Belgium. [Beyers, Koen; Vankerckhoven, Vanessa] Novosanis Nv, Wijnegem, Belgium. [Bruyndonckx, Robin] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, LMM, Fac Med & Hlth Sci, Antwerp, Belgium. [Bruyndonckx, Robin] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat I BIOST, Hasselt, Belgium. | Keywords: | Cervical cancer; First-void urine; Genotyping; HPV; Self-sampling;Cervical cancer; First-void urine; Genotyping; HPV; Self-sampling | Document URI: | http://hdl.handle.net/1942/28230 | ISSN: | 0166-0934 | e-ISSN: | 1879-0984 | DOI: | 10.1016/j.jviromet.2018.11.008 | ISI #: | 000456222300004 | Rights: | 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
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pattyn 1.pdf | Published version | 996.23 kB | Adobe PDF | View/Open |
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