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Title: | Isolation and Characterization of Lactobacillus brevisPhages. | Authors: | Feyereisen, Marine Mahony, Jennifer Lugli, Gabriele A. Ventura, Marco Neve, Horst Franz, Charles M.A.P. NOBEN, Jean-Paul O'Sullivan, Tadhg van Sinderen, Douwe |
Issue Date: | 2019 | Source: | Viruses, 11(5) | Abstract: | Lactobacillus brevis has been widely used in industry for fermentation purposes. However, it is also associated with the spoilage of foods and beverages, in particular, beer. There is an increasing demand for natural food preservation methods, and in this context, bacteriophages possess the potential to control such spoilage bacteria. Just a few studies on phages infecting Lactobacillus brevis have been performed to date and in the present study, we report the isolation and characterization of five virulent phages capable of infecting Lb. brevis strains. The analysis reveals a high diversity among the isolates, with members belonging to both, the Myoviridae and Siphoviridae families. One isolate, designated phage 3-521, possesses a genome of 140.8 kb, thus representing the largest Lb. brevis phage genome sequenced to date. While the isolated phages do not propagate on Lb. brevis beer-spoiling strains, phages showed activity against these strains, impairing the growth of some Lb. brevis strains. The results highlight the potential of bacteriophage-based treatments as an effective approach to prevent bacterial spoilage of beer. | Notes: | The authors are grateful to Dagmar Matoulková of the Research Institute of Brewing and Malting, Prague, who kindly provided Lb. brevis RIBM 2-56. The authors also acknowledge the technical help of Angela Back (Max Rubner-Institut, Kiel) in the electron microscopic analysis. van Sinderen, D (reprint author), Univ Coll Cork, Sch Microbiol, Cork T12 YT20, Ireland. Univ Coll Cork, APC Microbiome Ireland, Cork T12 YT20, Ireland. 116221209@umail.ucc.ie; J.Mahony@ucc.ie; gabrieleandrea.lugli@unipr.it; marco.ventura@unipr.it; horst.neve@mri.bund.de; charles.franz@mri.bund.de; jeanpaul.noben@uhasselt.be; Tadhg.OSullivan@heineken.com; d.vansinderen@ucc.ie | Keywords: | Lactobacillus brevis; bacteriophage; virulent; beer; fermentation; genomic and proteomic analysis | Document URI: | http://hdl.handle.net/1942/28346 | e-ISSN: | 1999-4915 | DOI: | 10.3390/v11050393 | ISI #: | 000472676600004 | Rights: | 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
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viruses-11-00393-v2.pdf | Published version | 2.8 MB | Adobe PDF | View/Open |
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