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http://hdl.handle.net/1942/28635
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DC Field | Value | Language |
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dc.contributor.author | DELLE, Lotta | - |
dc.contributor.author | Pachauri, Vivek | - |
dc.contributor.author | Sharma, Shikha | - |
dc.contributor.author | Shaforost, Olena | - |
dc.contributor.author | Ma, Hui | - |
dc.contributor.author | Adabi, Mohammad | - |
dc.contributor.author | Lilischkis, Rainer | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.contributor.author | THOELEN, Ronald | - |
dc.contributor.author | Klein, Norbert | - |
dc.contributor.author | O'Kennedy, Richard | - |
dc.contributor.author | Ingebrandt, Sven | - |
dc.date.accessioned | 2019-07-08T08:03:06Z | - |
dc.date.available | 2019-07-08T08:03:06Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, 102, p. 574-581 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/1942/28635 | - |
dc.description.abstract | Fatty-add binding proteins (FABP) and myeloperoxidases (MPO) are associated with many chronic conditions in humans and considered to be important biomarkers for diagnosis of cardiac diseases. Here we assemble a new electrical biosensor platform based on graphene-coated interdigitated electrode arrays (IDE-arrays) towards ultrafast, label-free screening of heart type-FABP and MPO. Arrays of nanoscale (nanoIDE) and microscale (microIDE) electrode-arrays were fabricated on wafer-scale by combining nanoimprint and photolithography processes. Chemical vapor deposition grown multilayer graphene was transferred onto nano/microIDE-arrays and used as a high surface-to-volume ratio electrical transducer. Novel biofunctional layers of specially engineered anti-h-FABP and anti-MPO single-chain fragment variables (scFv) were immobilized onto graphene-coated IDE-array sensor platform for electrical detection of h-FABP and MPO in physiological saline. scFv fragments show increased sensitivity in comparison to the state-of-the-art competitive ELISA for their higher affinity towards target analytes. Deploying FABP and MPO specific scFvs as receptor molecules onto our high sensitivity graphene-coated IDE-arrays with identical sensor characteristics and assays covering clinically relevant concentrations in physiological saline, we demonstrate realization of a simple and versatile biosensor platform capable of high performance cardiac-bioassays for point-of-care applications. | - |
dc.description.sponsorship | Stiftung Rheinland-Pfalz fur Innovation [1082]; Euroimmun AG; European Commission FP7 Programme through the Marie Curie Initial Training Network PROSENSE [317420]; DCU INVENT; Imperial sources | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject.other | scFV; Graphene; Biosensor; Cardiovascular; Electrical sensing; Physiological buffer | - |
dc.subject.other | scFV; Graphene; Biosensor; Cardiovascular; Electrical sensing; Physiological buffer | - |
dc.title | ScFv-modified graphene-coated IDE-arrays for 'label-free' screening of cardiovascular disease biomarkers in physiological saline | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 581 | - |
dc.identifier.spage | 574 | - |
dc.identifier.volume | 102 | - |
local.format.pages | 8 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Delle, Lotta E.; Pachauri, Vivek; Lilischkis, Rainer; Ingebrandt, Sven] Univ Appl Sci Kaiserslautern, Dept Informat & Microsyst Technol, Amerikastr 10, D-66482 Zweibrucken, Germany. [Sharma, Shikha; Ma, Hui; O'Kennedy, Richard] Dublin City Univ, Sch Biotechnol, Dublin 9, Ireland. [Shaforost, Olena; Adabi, Mohammad; Klein, Norbert] Imperial Coll London, Dept Mat, London SW7 2AZ, England. [Wagner, Patrick] Katholieke Univ Leuven, Dept Phys & Astron, Soft Matter Phys & Biophys Sect, Celestijnenlaan 200d, B-3001 Leuven, Belgium. [Thoelen, Ronald] Hassell Univ, Inst Mat Res, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. | - |
local.publisher.place | OXFORD | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.bios.2017.12.005 | - |
dc.identifier.isi | 000424176600076 | - |
item.validation | ecoom 2019 | - |
item.contributor | DELLE, Lotta | - |
item.contributor | Pachauri, Vivek | - |
item.contributor | Sharma, Shikha | - |
item.contributor | Shaforost, Olena | - |
item.contributor | Ma, Hui | - |
item.contributor | Adabi, Mohammad | - |
item.contributor | Lilischkis, Rainer | - |
item.contributor | WAGNER, Patrick | - |
item.contributor | THOELEN, Ronald | - |
item.contributor | Klein, Norbert | - |
item.contributor | O'Kennedy, Richard | - |
item.contributor | Ingebrandt, Sven | - |
item.accessRights | Open Access | - |
item.fullcitation | DELLE, Lotta; Pachauri, Vivek; Sharma, Shikha; Shaforost, Olena; Ma, Hui; Adabi, Mohammad; Lilischkis, Rainer; WAGNER, Patrick; THOELEN, Ronald; Klein, Norbert; O'Kennedy, Richard & Ingebrandt, Sven (2018) ScFv-modified graphene-coated IDE-arrays for 'label-free' screening of cardiovascular disease biomarkers in physiological saline. In: BIOSENSORS & BIOELECTRONICS, 102, p. 574-581. | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 0956-5663 | - |
crisitem.journal.eissn | 1873-4235 | - |
Appears in Collections: | Research publications |
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1-s2.0-S0956566317308011-main.pdf Restricted Access | Published version | 1.13 MB | Adobe PDF | View/Open Request a copy |
s1-ln251118795844769-1939656818Hwf-1569681356IdV18329150802511187PDF_HI0001.pdf | Peer-reviewed author version | 1.61 MB | Adobe PDF | View/Open |
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