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http://hdl.handle.net/1942/28728
Title: | Transcriptome profiling in blood before and after hepatitis B vaccination shows significant differences in gene expression between responders and non-responders | Authors: | Bartholomeus, Esther De Neuter, Nicolas Meysman, Pieter Suls, Arvid Keersmaekers, Nina Elias, George Jansens, Hilde HENS, Niel Smits, Evelien Van Tendeloo, Viggo Beutels, Philippe Van Damme, Pierre OGUNJIMI, Benson Laukens, Kris Mortier, Geert |
Issue Date: | 2018 | Publisher: | ELSEVIER SCI LTD | Source: | Vaccine, 36(42), p. 6282-6289 | Abstract: | Introduction: As the hepatitis B virus is widely spread and responsible for considerable morbidity and mortality, WHO recommends vaccination from infancy to reduce acute infection and chronic carriers. However, current subunit vaccines are not 100% efficacious and leave 5-10% of recipients unprotected. Methods: To evaluate immune responses after Engerix-B vaccination, we determined, using mRNA-sequencing, whole blood early gene expression signatures before, at day 3 and day 7 after the first dose and correlated this with the resulting antibody titer after two vaccine doses. Results: Our results indicate that immune related genes are differentially expressed in responders mostly at day 3 and in non-responders mostly at day 7. The most remarkable difference between responders and non-responders were the differentially expressed genes before vaccination. The granulin precursor gene (GRN) was significantly downregulated in responders while upregulated in non-responders at day 0. Furthermore, absolute granulocytes numbers were significantly higher in non-responders at day 0. Conclusion: The non-responders already showed an activated state of the immune system before vaccination. Furthermore, after vaccination, they exhibited a delayed and partial immune response in comparison to the responders. Our data may indicate that the baseline and untriggered immune system can influence the response upon hepatitis B vaccination. (C) 2018 Elsevier Ltd. All rights reserved. | Notes: | [Bartholomeus, Esther; Suls, Arvid; Mortier, Geert] Univ Antwerp, Antwerp Univ Hosp, Dept Med Genet, Prins Boudewijnlaan 43-6, B-2650 Edegem, Belgium. [Bartholomeus, Esther; De Neuter, Nicolas; Meysman, Pieter; Suls, Arvid; Keersmaekers, Nina; Elias, George; Hens, Niel; Smits, Evelien; Van Tendeloo, Viggo; Beutels, Philippe; Van Damme, Pierre; Ogunjimi, Benson; Laukens, Kris; Mortier, Geert] Univ Antwerp, Antwerp Unit Data Anal & Computat Immunol & Seque, AUDACIS, Antwerp, Belgium. [De Neuter, Nicolas; Meysman, Pieter; Laukens, Kris] Univ Antwerp, Dept Math & Comp Sci, Adrem Data Lab, Antwerp, Belgium. [De Neuter, Nicolas; Meysman, Pieter; Laukens, Kris] Univ Antwerp, Biomed Informat Res Network Antwerp Biomina, Antwerp, Belgium. [Keersmaekers, Nina; Hens, Niel; Beutels, Philippe; Ogunjimi, Benson] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, CHERMID, Antwerp, Belgium. [Elias, George; Smits, Evelien; Van Tendeloo, Viggo; Ogunjimi, Benson] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, LEH, Antwerp, Belgium. [Jansens, Hilde] Antwerp Univ Hosp, Dept Lab Med, Edegem, Belgium. [Hens, Niel] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Diepenbeek, Belgium. [Hens, Niel; Van Damme, Pierre] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, CEV, Antwerp, Belgium. [Smits, Evelien] Antwerp Univ Hosp, Ctr Cell Therapy & Regenerat Med, Edegem, Belgium. [Smits, Evelien] Univ Antwerp, Ctr Oncol Res Antwerp, Antwerp, Belgium. [Ogunjimi, Benson] Antwerp Univ Hosp, Dept Paediat, Edegem, Belgium. | Keywords: | Differential gene expression; Engerix-B vaccine; Hepatitis B; Immune response;differential gene expression; Engerix-B vaccine; hepatitis B; immune response | Document URI: | http://hdl.handle.net/1942/28728 | ISSN: | 0264-410X | e-ISSN: | 1873-2518 | DOI: | 10.1016/j.vaccine.2018.09.001 | ISI #: | 000447479200005 | Rights: | 2018 Elsevier Ltd. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
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bartholomeus 1.pdf Restricted Access | Published version | 862.61 kB | Adobe PDF | View/Open Request a copy |
Bartholomeus et al 2018 auteursversie.docx Restricted Access | Peer-reviewed author version | 104.12 kB | Microsoft Word | View/Open Request a copy |
Bartholomeus et al 2018 figures and links to supp mat.pdf Restricted Access | Supplementary material | 705.31 kB | Adobe PDF | View/Open Request a copy |
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