Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/28741
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | GUTSCH, Annelie | - |
dc.contributor.author | Zouaghi, Salha | - |
dc.contributor.author | Renaut, Jenny | - |
dc.contributor.author | CUYPERS, Ann | - |
dc.contributor.author | Hausman, Jean-Francois | - |
dc.contributor.author | Sergeant, Kjell | - |
dc.date.accessioned | 2019-07-16T14:28:26Z | - |
dc.date.available | 2019-07-16T14:28:26Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | International journal of molecular sciences, 19(9) (Art N° 2498) | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/1942/28741 | - |
dc.description.abstract | Accumulation of cadmium (Cd) shows a serious problem for the environment and poses a threat to plants. Plants employing various cellular and molecular mechanisms to limit Cd toxicity and alterations of the cell wall structure were observed upon Cd exposure. This study focuses on changes in the cell wall protein-enriched subproteome of alfalfa (Medicago sativa) leaves during long-term Cd exposure. Plants grew on Cd-contaminated soil (10 mg/kg dry weight (DW)) for an entire season. A targeted approach was used to sequentially extract cell wall protein-enriched fractions from the leaves and quantitative analyses were conducted with two-dimensional difference gel electrophoresis (2D DIGE) followed by protein identification with matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time of flight (TOF/TOF) mass spectrometry. In 212 spots that showed a significant change in intensity upon Cd exposure a single protein was identified. Of these, 163 proteins are predicted to be secreted and involved in various physiological processes. Proteins of other subcellular localization were mainly chloroplastic and decreased in response to Cd, which confirms the Cd-induced disturbance of the photosynthesis. The observed changes indicate an active defence response against a Cd-induced oxidative burst and a restructuring of the cell wall, which is, however, different to what is observed in M. sativa stems and will be discussed. | - |
dc.description.sponsorship | This work was supported by the FNR and FWO and is part of the bilateral project CadWALL (FNR/FWO project INTER/FWO/13/14). | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.rights | 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | - |
dc.subject.other | Medicago sativa; leaf cell wall proteome; cadmium; quantitative proteomics; 2D DIGE | - |
dc.subject.other | Medicago sativa; leaf cell wall proteome; cadmium; quantitative proteomics; 2D DIGE | - |
dc.title | Changes in the Proteome of Medicago sativa Leaves in Response to Long-Term Cadmium Exposure Using a Cell-Wall Targeted Approach | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 9 | - |
dc.identifier.volume | 19 | - |
local.format.pages | 17 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Gutsch, Annelie; Zouaghi, Salha; Renaut, Jenny; Hausman, Jean-Francois; Sergeant, Kjell] Luxembourg Inst Sci & Technol, Environm Res & Innovat, 5 Ave Hauts Fourneaux, L-4362 Luxembourg, Luxembourg. [Gutsch, Annelie; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, Agoralaan Bldg D,Campus Diepenbeek, B-3590 Diepenbeek, Belgium. | - |
local.publisher.place | BASEL | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 2498 | - |
dc.identifier.doi | 10.3390/ijms19092498 | - |
dc.identifier.isi | 000449988100034 | - |
item.fullcitation | GUTSCH, Annelie; Zouaghi, Salha; Renaut, Jenny; CUYPERS, Ann; Hausman, Jean-Francois & Sergeant, Kjell (2018) Changes in the Proteome of Medicago sativa Leaves in Response to Long-Term Cadmium Exposure Using a Cell-Wall Targeted Approach. In: International journal of molecular sciences, 19(9) (Art N° 2498). | - |
item.validation | ecoom 2019 | - |
item.accessRights | Open Access | - |
item.fulltext | With Fulltext | - |
item.contributor | GUTSCH, Annelie | - |
item.contributor | Zouaghi, Salha | - |
item.contributor | Renaut, Jenny | - |
item.contributor | CUYPERS, Ann | - |
item.contributor | Hausman, Jean-Francois | - |
item.contributor | Sergeant, Kjell | - |
crisitem.journal.issn | 1661-6596 | - |
crisitem.journal.eissn | 1422-0067 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
gutsch 1.pdf | Published version | 1.86 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
8
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
36
checked on Jul 13, 2024
Page view(s)
132
checked on Sep 6, 2022
Download(s)
150
checked on Sep 6, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.