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Title: | Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure | Authors: | Lermyte, Frederik VALKENBORG, Dirk Loo, Joseph A. Sobott, Frank |
Issue Date: | 2018 | Publisher: | WILEY | Source: | MASS SPECTROMETRY REVIEWS, 37(6), p. 750-771 | Abstract: | '] In recent years, electron capture (ECD) and electron transfer dissociation (ETD) have emerged as two of the most useful methods in mass spectrometry-based protein analysis, evidenced by a considerable and growing body of literature. In large part, the interest in these methods is due to their ability to induce backbone fragmentation with very little disruption of noncovalent interactions which allows inference of information regarding higher order structure from the observed fragmentation behavior. Here, we review the evolution of electron-based dissociation methods, and pay particular attention to their application in "native" mass spectrometry, their mechanism, determinants of fragmentation behavior, and recent developments in available instrumentation. Although we focus on the two most widely used methodsECD and ETDwe also discuss the use of other ion/electron, ion/ion, and ion/neutral fragmentation methods, useful for interrogation of a range of classes of biomolecules in positive- and negative-ion mode, and speculate about how this exciting field might evolve in the coming years. | Notes: | [Lermyte, Frederik; Sobott, Frank] Univ Antwerp, Dept Chem, Biomol & Analyt Mass Spectrometry Grp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. [Lermyte, Frederik; Valkenborg, Dirk] Univ Antwerp, Ctr Prote, Antwerp, Belgium. [Lermyte, Frederik] Univ Warwick, Sch Engn, Coventry, W Midlands, England. [Valkenborg, Dirk] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Agoralaan, Diepenbeek, Belgium. [Valkenborg, Dirk] Flemish Inst Technol Res VITO, Appl Bio & Mol Syst, Mol, Belgium. [Loo, Joseph A.] Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA. [Loo, Joseph A.] Univ Calif Los Angeles, Inst Genom & Prote, DOE, Los Angeles, CA USA. [Loo, Joseph A.] Univ Calif Los Angeles, Dept Chem & Biochem, 405 Hilgard Ave, Los Angeles, CA 90024 USA. [Sobott, Frank] Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds, W Yorkshire, England. [Sobott, Frank] Univ Leeds, Sch Mol & Cellular Biol, Leeds, W Yorkshire, England. | Keywords: | Selectron capture dissociation; electron transfer dissociation; noncovalent complex; proteinfolding; top-down fragmentation;electron capture dissociation; electron transfer dissociation; noncovalent complex; protein folding; top-down fragmentation | Document URI: | http://hdl.handle.net/1942/28830 | ISSN: | 0277-7037 | e-ISSN: | 1098-2787 | DOI: | 10.1002/mas.21560 | ISI #: | 000446306400003 | Rights: | 2018 Wiley Periodicals, Inc | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2019 |
Appears in Collections: | Research publications |
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