Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/28943
Title: | Regenerative Endodontic Procedures Posttrauma: Immunohistologic Analysis of a Retrospective Series of Failed Cases | Authors: | Meschi, Nastaran HILKENS, Petra Van Gorp, Gertrude Strijbos, Olaf Mavridou, Athina Perula, Maria Cadenas de Llano LAMBRICHTS, Ivo Verbeken, Eric Lambrechts, Paul |
Issue Date: | 2019 | Publisher: | ELSEVIER SCIENCE INC | Source: | JOURNAL OF ENDODONTICS, 45(4), p. 427-434 | Abstract: | Introduction: Regenerative endodontic procedures (REP) are a novel treatment modality to restore the function of necrotic pulp tissue via stimulation or transplantation of stem cells into the root canal. This study aimed to investigate the immunohistologic outcome of 3 extracted teeth because of sequelae of trauma and unsatisfactory REP outcomes. Methods: Three immature permanent maxillary central incisors of 3 female patients (6-9 years) were extracted 5.5-22 months after REP. Additionally, 1 sound permanent immature central maxillary incisor of 1 of the included patients was extracted for orthodontic reasons. The teeth were immunohistologically stained with Masson's trichrome, neurofilament (NF), pan cytokeratin, dentin sialophos-phoprotein, and Gram+/-. Results: The REP-teeth pre- sented intracanalar vascularized connective/mineralized reparative tissue (RT), which was less organized than the pulp tissue of the sound tooth. Moderate to considerable calcification was observed below the Portland cement used during REP. In 1 case, the RT was NF+; in the 2 other cases, the periodontal ligament and apical granuloma/papilla were NF+. All teeth were Gram+/- negative; nevertheless, inflammatory cells were present in 2 cases. The pan cytokeratin and dentin sialophosphoprotein stainings were not specific enough for 2 cases. Conclusions: This immunohistologic study of failed REP cases resulted in bacteria-free intracanalar RT and biomaterial-induced calcification. Nevertheless, the presence of inflammatory cells revealed a persistent inflammation. Hence, the clinical and radiographic signs were decisive for tooth survival and multidisciplinary outcome determination. | Notes: | [Meschi, Nastaran; Van Gorp, Gertrude; Strijbos, Olaf; Mavridou, Athina; Lambrechts, Paul] Katholieke Univ Leuven, Dept Oral Hlth Sci, Endodontol, Leuven, Belgium. [Perula, Maria Cadenas de Llano] Katholieke Univ Leuven, Dept Oral Hlth Sci, Orthodont, Leuven, Belgium. [Verbeken, Eric] Katholieke Univ Leuven, Dept Translat Cell & Tissue Res, Leuven, Belgium. [Meschi, Nastaran; Mavridou, Athina; Lambrechts, Paul] BIOMAT, Biomat, Kapucijnenvoer 7 Blok A,Box 7001, B-3000 Leuven, Belgium. [Hilkens, Petra; Lambrichts, Ivo] Hasselt Univ, Biomed Res Inst, Lab Morphol, Diepenbeek, Belgium. | Keywords: | Angiogenesis; cone-beam computed tomography; dental pulp; histology; stem cell(s);Angiogenesis; cone-beam computed tomography; dental pulp; histology; stem cell(s) | Document URI: | http://hdl.handle.net/1942/28943 | ISSN: | 0099-2399 | e-ISSN: | 1878-3554 | DOI: | 10.1016/j.joen.2019.01.007 | ISI #: | 000464300900010 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
meschi 1.pdf Restricted Access | Published version | 2.43 MB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
2
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
21
checked on Apr 24, 2024
Page view(s)
146
checked on Aug 31, 2022
Download(s)
124
checked on Aug 31, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.