Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/28981
Title: Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?
Authors: GUTSCH, Annelie 
Sergeant, Kjell
KEUNEN, Els 
Prinsen, Els
Guerriero, Gea
Renaut, Jenny
Hausman, Jean-Francois
CUYPERS, Ann 
Issue Date: 2019
Publisher: BMC
Source: BMC PLANT BIOLOGY, 19 (Art N° 271)
Abstract: BackgroundThe heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure. In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd. Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.ResultsSeveral proteins determining for the cell wall structure changed in abundance. Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd. Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.ConclusionsAn increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone. Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.
Notes: [Gutsch, Annelie; Sergeant, Kjell; Guerriero, Gea; Renaut, Jenny; Hausman, Jean-Francois] Luxembourg Inst Sci & Technol, Environm Res & Innovat Dept, 5 Ave Hauts Fourneaux, L-4362 Esch Sur Alzette, Luxembourg. [Gutsch, Annelie; Keunen, Els; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Prinsen, Els] Univ Antwerp, Dept Biol, Integrated Mol Plant Res, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
Keywords: Long-term cadmium exposure; Medicago sativa; Label-free protein quantification; Gene expression; Cell wall; Lignin; Pectin methylesterase;Long-term cadmium exposure; Medicago sativa; Label-free protein quantification; Gene expression; Cell wall; Lignin; Pectin methylesterase
Document URI: http://hdl.handle.net/1942/28981
ISSN: 1471-2229
e-ISSN: 1471-2229
DOI: 10.1186/s12870-019-1859-y
ISI #: 000472489300003
Rights: The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Category: A1
Type: Journal Contribution
Validations: ecoom 2020
Appears in Collections:Research publications

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