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http://hdl.handle.net/1942/29032
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DC Field | Value | Language |
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dc.contributor.author | Birgit, Stubbe | - |
dc.contributor.author | GRAULUS, Geert-Jan | - |
dc.contributor.author | REEKMANS, Gunter | - |
dc.contributor.author | Courtin, Timothee | - |
dc.contributor.author | Martins, José C. | - |
dc.contributor.author | Van Vlierberghe, Sandra | - |
dc.contributor.author | Dubruel, Peter | - |
dc.contributor.author | ADRIAENSENS, Peter | - |
dc.date.accessioned | 2019-08-26T07:58:15Z | - |
dc.date.available | 2019-08-26T07:58:15Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | International journal of biological macromolecules, 134, p. 722-729 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | http://hdl.handle.net/1942/29032 | - |
dc.description.abstract | Alginates are fairly abundant in nature and possess many interesting properties, including their biocompatibility and ability to absorb large amounts of water. Hence, increasing interest in their derivatization has been observed and the determination of the number of newly introduced functionalities has become a key issue. For this purpose, literature generally reports on conventional 1H‐NMR spectra, typically recorded at elevated temperatures and/or after hydrolysis of the alginate to circumvent line broadening effects resulting from the high viscosity. The present work reports on the modification of alginate with methacrylate functionalities and determination of the resulting degree of substitution (DS), i.e. the number of introduced methacrylate moieties relative to the initial amount of hydroxyl groups along the alginate backbone, via NMR spectroscopy. Freeze-drying and low power water presaturation were applied to improve the quality of the 1H NMR spectra. Nevertheless, it remains a qualitative method, to be used only for mutual comparisons of samples. A new and accurate method for DS determination of methacrylated alginates, based on 13C‐NMR spectroscopy, is proposed. Quantitative 13C‐NMR spectra were recorded with reduced measuring times by addition of a paramagnetic relaxation agent. The proposed method will also be applicable for other water-soluble functionalized alginates and polysaccharides in general. | - |
dc.description.sponsorship | The authors wish to acknowledge the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT) for the Ph.D. funding granted to B. Stubbe. Geert-Jan Graulus, Dr. Krisztina Feher and Tim Courtin are acknowledged for their assistance with the recording of HR-MAS 1H NMR spectra. J. C. Martins and P. Adriaensens want to thank the Research Foundation Flanders (FWO-Vlaanderen) for the support of exchanges via the MULTIMAR project The authors would like to acknowledge Ghent University for financial support in the frameworks of the UGent-GOA project 2010-2015 (BOF10/GOA/005, Biomedical Engineering for Improved Diagnosis and Patient-Tailored Treatment of Aortic Aneurysms and Dissection) and the UGent Multidisciplinary Research Partnership Nano-and biophotonics (2010-2014). Sandra Van Vlierberghe would like to acknowledge the Research Foundation Flanders (FWO, Belgium) for financial support under the form of a post-doctoral fellowship and a Research Grant ('Development of the ideal tissue engineering scaffold by merging state-of-the-art processing techniques', FWO Krediet aan Navorsers). The 700 MHz NMR equipment used in this work is part of the Interuniversity NMR Facility and was funded through a FFEU-ZWAP grant from the Flemish Government. | - |
dc.language.iso | en | - |
dc.rights | 2019 Elsevier B.V. All rights reserved | - |
dc.subject.other | Functionalized alginate; Degree of substitution; NMR spectroscopy | - |
dc.title | A straightforward method for quantification of vinyl functionalized water soluble alginates via 13C-NMR spectroscopy | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 729 | - |
dc.identifier.spage | 722 | - |
dc.identifier.volume | 134 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Dubruel, P (reprint author), Krijgslaan 281 Bldg S4 Bis, B-9000 Ghent, Belgium. Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. Peter.Dubruel@ugent.be; Peter.Adriaensens@UHasselt.le | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2019.05.015 | - |
dc.identifier.isi | 000474673600076 | - |
item.validation | ecoom 2020 | - |
item.contributor | Birgit, Stubbe | - |
item.contributor | GRAULUS, Geert-Jan | - |
item.contributor | REEKMANS, Gunter | - |
item.contributor | Courtin, Timothee | - |
item.contributor | Martins, José C. | - |
item.contributor | Van Vlierberghe, Sandra | - |
item.contributor | Dubruel, Peter | - |
item.contributor | ADRIAENSENS, Peter | - |
item.accessRights | Restricted Access | - |
item.fullcitation | Birgit, Stubbe; GRAULUS, Geert-Jan; REEKMANS, Gunter; Courtin, Timothee; Martins, José C.; Van Vlierberghe, Sandra; Dubruel, Peter & ADRIAENSENS, Peter (2019) A straightforward method for quantification of vinyl functionalized water soluble alginates via 13C-NMR spectroscopy. In: International journal of biological macromolecules, 134, p. 722-729. | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 0141-8130 | - |
crisitem.journal.eissn | 1879-0003 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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Stubbe 2019 .pdf Restricted Access | Published version | 1.24 MB | Adobe PDF | View/Open Request a copy |
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