Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/29058
Title: | Simultaneous determination of parabens, bisphenols and alkylphenols in human placenta by ultra-high performance liquid chromatography-tandem mass spectrometry | Authors: | Van Overmeire, Ilse VRIJENS, Karen NAWROT, Tim Van Nieuwenhuyse, An Van Loco, Joris Reyns, Tim |
Issue Date: | 2019 | Publisher: | ELSEVIER SCIENCE BV | Source: | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1121, p. 96-102 | Abstract: | This study presents de development and validation of an ultra-high performance liquid chromatography - tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous determination of four parabens (methyl-, ethyl-, propyl-, and butyl-paraben (MeP, EtP, PrP, BuP), four bisphenols (BP) (BPA, BPB, BPF, and BPS) and two alkylphenols (nonyl phenol (NP) and tert-octylphenol (OP) in human placenta samples. After a short sample preparation time the extracts are analysed by UHPLC-MS/MS using negative electrospray ionization. Labeled internal standards and matrix-matched calibration are used for quantification of the compounds. The method was validated according FDA guideline for bio analytical methods using spiked samples at three concentration levels (0.5-5 and 25 ng g(-1)). The parameters accuracy and precision fulfill the criteria. Calibration curves are linear between 0.5 and 50 ng (-1). The limits of detection and quantification are in the range of 0.1-0.3 ng g(-1) and 0.2-0.7 ng g(-1), respectively. The applicability of the method was demonstrated on 71 human placenta samples from a Belgian cohort. The detection frequency was highest for OP (95%), EtP (86%), BPA (49%) and BPS (44%). Among the quantified compounds the highest quantification frequency was observed for OP (85%), EtP (65%) and BPA (25%). The concentrations of parabens ranged from 0.5 to 7.1 ng g(-1) for MeP, from 0.5 to 4.5 ng g(-1) for EtP and from 0.5 to 9.1 ng g(-1) for PrP. The levels of bisphenols ranged from 0.5 to 3.9 ng g(-1) for BPA, from 0.6 to 2.1 ng g(-1) for BPF and from 0.8 to 1.3 ng g(-1) for BPS. BPB and NP were not detected and OP levels ranged from 0.5 to 3.7 ng g g(-1). The results demonstrate that the developed analytical method is very sensitive and that levels of several compounds with known /suspected endocrine disrupting properties could be detected or quantified in human placenta samples. The results therefore suggest that fetal exposure to these compounds occurs. The method will be useful for studies to evaluate the health effects associated with this prenatal exposure. | Notes: | [Van Overmeire, Ilse; Van Nieuwenhuyse, An; Van Loco, Joris; Reyns, Tim] Sciensano, Chem & Phys Hlth Risks, B-1050 Brussels, Belgium. [Vrijens, Karen; Nawrot, Tim] Hasselt Univ, Ctr Environm Sci, B-3500 Hasselt, Belgium. [Nawrot, Tim] Leuven Univ, KU Leuven, Dept Publ Hlth, Environm & Hlth Unit, B-3000 Leuven, Belgium. | Keywords: | Placenta; Endocrine;disruptor; UHPLC-MS/MS; Bisphenol; Paraben; Alkylphenol;Placenta; Endocrine disruptor; UHPLC-MS/MS; Bisphenol; Paraben; Alkylphenol | Document URI: | http://hdl.handle.net/1942/29058 | ISSN: | 1570-0232 | e-ISSN: | 1873-376X | DOI: | 10.1016/j.jchromb.2019.05.012 | ISI #: | 000471207200012 | Rights: | 2019 Elsevier B.V. All rights reserved | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
van overmeire 1.pdf Restricted Access | Published version | 574.18 kB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
1
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
18
checked on Mar 29, 2024
Page view(s)
124
checked on Sep 7, 2022
Download(s)
112
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.