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http://hdl.handle.net/1942/29293
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DC Field | Value | Language |
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dc.contributor.advisor | THOELEN, Ronald | - |
dc.contributor.author | Zain, Ashwaq | - |
dc.date.accessioned | 2019-09-17T08:27:19Z | - |
dc.date.available | 2019-09-17T08:27:19Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | http://hdl.handle.net/1942/29293 | - |
dc.description.abstract | Molecular diagnostics are very attractive tools for pathogen detection. But, the conventional molecular diagnostics are unsuitable for poor point of care (POC). So, miniaturizing lab devices towards a Lab-on-card (LOC) is useful in POC. We aimed to translate macro-lab techniques to a single LOC sensor for cell lysis & DNA extraction, amplification & detection using Loop-Mediated isothermal amplification (LAMP). LAMP is sensitive, cheap, rapid and very appealing for POC. So, we hypothesized “LAMP can be incorporated for the development of a LOC sensor for DNA extraction, amplification and detection”.The next goals were tested on card: cell extraction, cell lysis & DNA extraction, mixing samples &reagents, and optical DNA detection. A card was designed to test each step separately. Artificial sputum is prepared & liquified by hot water. Cells were extracted by filter paper. Thermal cell lysis was tested by microheater & DNA extraction was estimated by Nanodrop. A micromixer is tested to mix the reagents. The detection of the DNA amplicons was performed by a photosensor on card. Here we provided cards that can, extract cells in sputum, rupture cells and extract DNA thermally, mix sample and reagents, detect the DNA by a photosensor. Each step was tested on card separately.To conclude, each step of the DNA sensor was performed by LOC. Hence, the development of a single DNA card sensor is very promising which is of great value in POC & remote settings | - |
dc.format.mimetype | Application/pdf | - |
dc.language | en | - |
dc.publisher | tUL | - |
dc.title | Lab-on-card based sensor for DNA detection using Loop-mediated isothermal amplification | - |
dc.type | Theses and Dissertations | - |
local.format.pages | 0 | - |
local.bibliographicCitation.jcat | T2 | - |
dc.description.notes | Master of Biomedical Sciences-Environmental Health Sciences | - |
local.type.specified | Master thesis | - |
item.fullcitation | Zain, Ashwaq (2019) Lab-on-card based sensor for DNA detection using Loop-mediated isothermal amplification. | - |
item.fulltext | With Fulltext | - |
item.contributor | Zain, Ashwaq | - |
item.accessRights | Open Access | - |
Appears in Collections: | Master theses |
Files in This Item:
File | Description | Size | Format | |
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551122ee-5287-4257-a2a6-2a31fefb821e.pdf | 1.98 MB | Adobe PDF | View/Open |
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