Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/29551
Title: | Genomic Diversity of Two Hydrocarbon-Degrading and Plant Growth-Promoting Pseudomonas Species Isolated from the Oil Field of Bóbrka (Poland) | Authors: | IMPERATO, Valeria Portillo-Estrada, Miguel McAmmond, M. Breanne DOUWEN, Yorben Van Hamme, Jonathan D. Gawronski, Stanislaw W. VANGRONSVELD, Jaco THIJS, Sofie |
Issue Date: | 2019 | Source: | Genes, 10(6) (Art N° 443) | Abstract: | Hydrocarbon-degrading bacteria are important resources for use in phytoremediation applications. Yet, for many hydrocarbonoclastic strains the genetic information regarding pollutant degradation and detoxification has not been thoroughly revealed. In this study, hydrocarbon-degrading bacteria were isolated from a long-term oil-polluted soil in Bóbrka, Poland. Pseudomonas spp. was the most dominant species. Of all 69 isolated strains tested in the laboratory using qualitative biochemical assays, 61% showed the capability to use diesel as sole carbon source, 33% could produce indole, 19% produced siderophores, 36% produced organic acids, and 54% were capable of producing 1-aminocyclopropane-1-carboxylate (ACC)-deaminase. From all morphologically and genetically different strains, two representative Pseudomonas spp., strain VI4.1 and VI4T1, were selected for genome sequencing. Genomic analyses indicated the presence of the full naphthalene dioxygenase operon (plasmid and chromosomal), of genes involved in the degradation of BTEX compounds (Benzene, Toluene, Ethylbenzene, Xylene) and alkanes (alkB gene) as well as the anthranilate degradation pathway (strain VI4T1) and terephthalate dioxygenase protein (strain VI4.1). Proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) analyses confirmed naphthalene and BTEX degradation within seven days. Motility, resistance to abiotic stresses, high and low temperatures, low pH, and salinity were confirmed at the genetic level and experimentally verified. The presence of multiple degradative and plant growth promotion genes, together with the in vitro experimental evidence, indicates the high value of these two strains and their potential use for sustainable site clean-up. | Keywords: | naphthalene; toluene; hydrocarbons; plant growth promotion; bioremediation; Pseudomonas; soil pollution; phytoremediation | Document URI: | http://hdl.handle.net/1942/29551 | e-ISSN: | 2073-4425 | DOI: | 10.3390/genes10060443 | ISI #: | 000473797000038 | Rights: | 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
genes-10-00443 (1).pdf | Published version | 6.45 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
5
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
26
checked on Apr 23, 2024
Page view(s)
128
checked on Sep 7, 2022
Download(s)
164
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.