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Title: | The nitric oxide suppressed Arabidopsis mutants-Atnoa1 and Atnia1nia2noa1-2 produce nitric oxide in MS growth medium and on uranium exposure | Authors: | Tewari, Rajesh Kumar HOREMANS, Nele Nauts, Robin Wannijn, Jean Van Hees, May VANDENHOVE, Hildegarde |
Issue Date: | 2019 | Publisher: | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | Source: | PLANT PHYSIOLOGY AND BIOCHEMISTRY, 140, p. 9-17 | Abstract: | The mutants Atnoa1 and Atnia1nia2noa1-2 having a defective chloroplast developmental process, showed enhanced chlorophyll levels when they were grown on Murashige and Skoog (MS) medium and on exposure with uranium (U) on Hoagland medium. Thus we hypothesized that these mutants probably produced NO in MS medium and on exposure with U. Wild-type Col-0, Atnoa1, Atnia1nia2noa1-2 plants were cultured on modified Hoagland and 1/10 MS media and NO generation in the roots of these mutants was monitored using NO selective fluorescent dyes, DAF-2DA and F12E. Both Atnoa1 and Atnia1nia2noa1-2 triple mutants produced NO as observed by increases in DAF-2T and F12E fluorescence when these mutants were grown on MS medium but not on Hoagland medium. In presence of NO scavenger, methylene blue (MB, 200 mu M), DAF-2T and F12E fluorescence was completely abolished. On the other hand treatment of the plants with 25 mu M U triggered NO generation. Utreated Atnoa1 and Atnia1nia2noa1-2 plants upregulated genes (POR B, POR D, CHL D) involved in the chlorophyll biosynthesis. From these results it was concluded that Atnoa1 and Atnia1nia2noa1-2 are conditional NO producers and it appears that NO generation in plants substantially depends on growth medium and NIA1, NIA2 or NOA1 does not appear to be really involved in NO generation in MS medium or after U exposure. | Notes: | [Tewari, Rajesh Kumar; Nauts, Robin] Univ Lucknow, Dept Bot, Lucknow 226007, Uttar Pradesh, India. [Tewari, Rajesh Kumar; Horemans, Nele; Wannijn, Jean; Van Hees, May; Vandenhove, Hildegarde] Belgian Nucl Res Ctr SCK CEN, Biosphere Impact Studies, Boeretang 200, B-2400 Mol, Belgium. [Horemans, Nele] Hasselt Univ, Ctr Environm Sci, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. | Keywords: | Atnoa1; Atnia1nia2noa1-2; Hoagland medium; MS medium; Nitric oxide; Uranium;Atnoa1; Atnia1nia2noa1-2; Hoagland medium; MS medium; Nitric oxide; Uranium | Document URI: | http://hdl.handle.net/1942/29640 | ISSN: | 0981-9428 | e-ISSN: | 1873-2690 | DOI: | 10.1016/j.plaphy.2019.04.042 | ISI #: | 000471736300002 | Rights: | 2019 Elsevier Masson SAS. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
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