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Title: | Difference in Pathomechanism Between Crohn's Disease and Ulcerative Colitis Revealed by Colon Transcriptome | Authors: | Yang, Lili Tang, Shijie Baker, Susan S. ARIJS, Ingrid Liu, Wensheng Alkhouri, Razan Lan, Ping Baker, Robert D. Tang, Zhipeng Ji, Guang Rutgeerts, Paul Vermeire, Severine Zhu, Ruixin Zhu, Lixin |
Issue Date: | 2019 | Publisher: | OXFORD UNIV PRESS INC | Source: | INFLAMMATORY BOWEL DISEASES, 25(4), p. 722-731 | Abstract: | Background: We aim to identify the differences in colonic mucosal transcriptome between Crohn's disease (CD) and ulcerative colitis (UC) for a better understanding of the molecular pathology. Methods: Differentially expressed genes (DEG) in the colonic mucosa of CD and UC were identified with a global gene expression microarray dataset generated from the colon biopsies of CD and UC patients and normal controls. The DEGs were then processed to identify altered pathways and modularized DEGs and pathways. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis with an independent cohort of samples was performed to validate the microarray data. Results: At the pathway level, virus infection and autoimmune pathways were upregulated in CD but not in UC when compared with controls. Some of the relevant DEGs (such as TAP1 and TAP2) were elevated in both CD and UC, with CD exhibiting more pronounced elevations. Gene expression levels in viral infection pathways were correlated with those of autoimmune pathways. In contrast, pattern recognition-mediated innate immune pathways (TLR4 and TLR2) were significantly elevated in UC but not in CD. Similar results were observed with an independent cohort by qRT-PCR. Conclusions: Our data support the hypothesis that viral infection induced autoimmunity may represent a pathomechanism for IBD, especially CD. However, pattern recognition-mediated innate immunity targeting microbiome may play a more important role in UC compared with CD. Our findings identified different intervention targets for CD and UC, which may lead to more effective treatments for IBD patients. | Notes: | [Yang, Lili; Tang, Zhipeng; Ji, Guang; Zhu, Lixin] Shanghai Univ Tradit Chinese Med, LongHua Hosp, Inst Digest Dis, Shanghai, Peoples R China. [Yang, Lili; Baker, Susan S.; Liu, Wensheng; Alkhouri, Razan; Baker, Robert D.; Zhu, Lixin] SUNY Buffalo, Women & Childrens Hosp Buffalo, Digest Dis & Nutr Ctr, Dept Pediat, Buffalo, NY USA. [Tang, Shijie; Zhu, Ruixin] Tongji Univ, Dept Bioinformat, Shanghai, Peoples R China. [Arijs, Ingrid; Rutgeerts, Paul; Vermeire, Severine] Katholieke Univ Leuven, Dept Clin & Expt Med, Translat Res Ctr Gastrointestinal Disorders TARGI, Leuven, Belgium. [Arijs, Ingrid] Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium. [Arijs, Ingrid] Jessa Hosp, Hasselt, Belgium. [Lan, Ping; Zhu, Lixin] Sun Yat Sen Univ, Affiliated Hosp 6, Guangdong Inst Gastroenterol, Guangdong Prov Key Lab Colorectal & Pelv Floor Di, Guangzhou, Guangdong, Peoples R China. [Baker, Susan S.; Zhu, Lixin] SUNY Buffalo, Genome Environm & Microbiome Community Excellence, Buffalo, NY 14214 USA. | Keywords: | Crohn's disease; ulcerative colitis; autoimmune; viral infection; TLR4;Crohn’s disease; ulcerative colitis; autoimmune; viral infection; TLR4 | Document URI: | http://hdl.handle.net/1942/29648 | ISSN: | 1078-0998 | e-ISSN: | 1536-4844 | DOI: | 10.1093/ibd/izy359 | ISI #: | 000473611900023 | Rights: | Crohn’s & Colitis Foundation. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2020 |
Appears in Collections: | Research publications |
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